Label three sets of eight microfuge tubes (1-8) and add the volumes of BSA stock solution (2 mg/ml) and water to each labeled tube as indicated in Table 3. Be sure to calculate, and enter, the values for column 4 of this table. Table 3 STD Curve Volume BSA Volume Water (µ) Final BSA Concentration tube (mg/ml) Stock (ul) number 100 2 5 95 3 10 90 4 20 80 5 40 60 6 60 40 7 80 20 100 00
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- The patient is ordered 150,000 units of Pfizerpen. Using 10mL of diluent, how many mL(s) will you administer? If rounding is requiredm, round to the nearest tenth. (Image for question is attached for more information)Using C1*V1 = C2*V2, please complete the calculations for the substrate row & show me how to do it as I am unsure. *The final sample is contained in using 2mL centrifuge tubes. answer all or else I will upvoteYou have prepared the following serial dilution using tryptone broth:d1: 0.1ml culture + 9.9 ml brothd2: 300µl d1 + 4.7 ml brothd3: 0.2ml d2 + 9800 µl broth1) Write your dilutions as a rational number AND in scientific format? What was your total dilution (in scientific notation)?2) What was the original concentration of your culture if you spread 0.1 ml of d3 and counted360 cfu?
- In the spread plate method, why is the volume plated usually limited to not more than 0.1 mL?How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Question: What is the distance of one ocular division? Show your solution.
- You are asked to prepare Murashige & Skoog (MS) medium according to concentration shown the Table. Answer the following question: 1. Prepare 250 mL stock solution (200X more concentrated) of ammonium nitrate and KNO3. 2. From the prepared ammonium nitrate stock solution above, calculate the volume required to prepare 200 mL MS medium.Prepare 50 plates of Sheep's Blood Agar using 100mm petri dish. Add 10% of blood to the media. (Manufacturer's instructions: Suspend 38 g of blood agar base dehydrated powder in 1 liter of distilled or deionized water.) Pour 25 mL of prepared medium per plate. Express answer to the nearest whole number. How many grams of the dehydrated powder should be weighed? Answer: What is the volume of distilled water that should be used in the preparation? Answer: What is the volume of sheep's blood that should be added to the sterilized and cooled blood agar base? Answer:How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? V1= 0.005mL B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?
- Observe the following Plate counts and then determine the correct number of CFU/ml Plate 1 = 564 colonies at 10^-5 dilution Plate 2 = 422 colonies at 10^-6 dilution Plate 3 = 317 colonies at 10^-7 dilution Plate 4 = 93 colonies at 10^-8 dilution 93 x 10^10 CFU/ml 9.3 x 10^-9 CFU/ml 93 x 10^9 CFU/ml 93x 10^8 CFÜ/ml 93x 10^-8 CFU/ml asap pleaseGiven: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Question: What is the total magnification of the image under microscope? Show your solution.Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Questions: What is the total magnification of the image under microscope? Show your solution. What is the distance of one ocular division? Show your solution. What is approximate cell length of Cell A, Cell B, and Cell C? What is approximate combine cell length of Cell A and Cell B? Show your solution. Remember: Always measure the cell length on the longest middle section of the cell!