Linkage Mapping Using a Trihybrid Testcross in Fruit Flies Remember the black, purple, and vestigial genes from Exercise 1? Now you're going to have to construct a map of the same region from trihybrid testcross data. The new map should be similar to the one from Exercise 1, but it won't be identical because we're starting with a new, independent data set. BLACK PURPLE VESTIGIAL TRIHYBRID TESTCROSS In the parental generation, you mate a pure-breeding wild-type female (bl*/bl*.pu*/pu vg+/vg+t with a pure-breeding black, purple, vestigial male (bl/bl pu/pu vg/vg) to produce an F1 generation that is all wild-type (bl*/bl pu*/pu.vg+/vg). Note that the F1 flies are all trihybrid. Next, you mate several F1 trihybrid females (blt/bl:put/pu vg+/vg) with tester males, which are black, purple, vestigial (bl/bl pu/pu.vg/vg). The offspring of this trihybrid testcross are: Phenotype Wild-type Black, purple, vestigial Vestigial Black, purple Purple, vestigial Black Purple Black, vestigial Genotype +/bl;+/pu;+/vg bl/bl;pu/pu;vg/vg +/bl;+/pu;vg/vg bl/bl:pu/pu;+/vg +/bl;pu/pu;vg/vg bl/bl:+/pu;+/vg +/bl; pu/pu;+/vg bl/bl;+/pu;vg/vg Tester Gamete Trihybrid Gamete Number bl pu vg 2332 2333 bl pu vg bi pu vg 338 bl pu vg 337 151 bl pu vg 171 bi pu vg bl pu vg 24 29 bl pu vg +++ bl pu vg ++vg bl pu+ + pu vg bl ++ + pu+ bl+vg D of that you downloaded in

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Chapter6: Cytogenetics: Karyotypes And Chromosome Aberrations
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Linkage Mapping Using a Trihybrid Testcross in Fruit Flies
Remember the black, purple, and vestigial genes from Exercise 1? Now you're going to have to construct a map of the same region from
trihybrid testcross data. The new map should be similar to the one from Exercise 1, but it won't be identical because we're starting with a
new, independent data set.
BLACK PURPLE VESTIGIAL TRIHYBRID TESTCROSS
In the parental generation, you mate a pure-breeding wild-type female (bl+/bl*pu*/pu+vg+/vg+ with a pure-breeding black, purple,
vestigial male (bl/bl;pu/pu vg/vg) to produce an F1 generation that is all wild-type (bl/bliput/pu;vg+/vg). Note that the F1 flies are all
trihybrid.
Next, you mate several F1 trihybrid females (bl/bl:put/pu;vg+/vg) with tester males, which are black, purple, vestigial (bl/bl;pu/pu;vg/vg).
The offspring of this trihybrid testcross are:
Phenotype
Wild-type
Black, purple, vestigial
Vestigial
Black, purple
Purple, vestigial
Black
Purple
Black, vestigial
bl pu vg
bl pu vg
Genotype Tester Gamete Trihybrid Gamete Number
+/bl;+/pu;+/vg
bl pu vg
bl/bl;pu/pu;vg/vg
+/bl;+/pu;vg/vg
bl/bl;pu/pu;+/vg
+/bl;pu/pu;vg/vg
bl/bl;+/pu;+/vg
+/bl; pu/pu;+/vg
bl/bl;+/pu;vg/vg
bl pu vg
bl pu vg
bl pu vg
bl pu vg
bl pu vg
+++
bl pu vg
+ + vg
bl pu+
+ pu vg
bl ++
+ pu +
bl + vg
2332
2333
338
337
151
171
24
29
A
the questions in the worksheet that you downloaded in
Transcribed Image Text:Linkage Mapping Using a Trihybrid Testcross in Fruit Flies Remember the black, purple, and vestigial genes from Exercise 1? Now you're going to have to construct a map of the same region from trihybrid testcross data. The new map should be similar to the one from Exercise 1, but it won't be identical because we're starting with a new, independent data set. BLACK PURPLE VESTIGIAL TRIHYBRID TESTCROSS In the parental generation, you mate a pure-breeding wild-type female (bl+/bl*pu*/pu+vg+/vg+ with a pure-breeding black, purple, vestigial male (bl/bl;pu/pu vg/vg) to produce an F1 generation that is all wild-type (bl/bliput/pu;vg+/vg). Note that the F1 flies are all trihybrid. Next, you mate several F1 trihybrid females (bl/bl:put/pu;vg+/vg) with tester males, which are black, purple, vestigial (bl/bl;pu/pu;vg/vg). The offspring of this trihybrid testcross are: Phenotype Wild-type Black, purple, vestigial Vestigial Black, purple Purple, vestigial Black Purple Black, vestigial bl pu vg bl pu vg Genotype Tester Gamete Trihybrid Gamete Number +/bl;+/pu;+/vg bl pu vg bl/bl;pu/pu;vg/vg +/bl;+/pu;vg/vg bl/bl;pu/pu;+/vg +/bl;pu/pu;vg/vg bl/bl;+/pu;+/vg +/bl; pu/pu;+/vg bl/bl;+/pu;vg/vg bl pu vg bl pu vg bl pu vg bl pu vg bl pu vg +++ bl pu vg + + vg bl pu+ + pu vg bl ++ + pu + bl + vg 2332 2333 338 337 151 171 24 29 A the questions in the worksheet that you downloaded in
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