Linkage Mapping Using a Trihybrid Testcross in Fruit Flies Remember the black, purple, and vestigial genes from Exercise 1? Now you're going to have to construct a map of the same region from trihybrid testcross data. The new map should be similar to the one from Exercise 1, but it won't be identical because we're starting with a new, independent data set. BLACK PURPLE VESTIGIAL TRIHYBRID TESTCROSS In the parental generation, you mate a pure-breeding wild-type female (bl*/bl*.pu*/pu vg+/vg+t with a pure-breeding black, purple, vestigial male (bl/bl pu/pu vg/vg) to produce an F1 generation that is all wild-type (bl*/bl pu*/pu.vg+/vg). Note that the F1 flies are all trihybrid. Next, you mate several F1 trihybrid females (blt/bl:put/pu vg+/vg) with tester males, which are black, purple, vestigial (bl/bl pu/pu.vg/vg). The offspring of this trihybrid testcross are: Phenotype Wild-type Black, purple, vestigial Vestigial Black, purple Purple, vestigial Black Purple Black, vestigial Genotype +/bl;+/pu;+/vg bl/bl;pu/pu;vg/vg +/bl;+/pu;vg/vg bl/bl:pu/pu;+/vg +/bl;pu/pu;vg/vg bl/bl:+/pu;+/vg +/bl; pu/pu;+/vg bl/bl;+/pu;vg/vg Tester Gamete Trihybrid Gamete Number bl pu vg 2332 2333 bl pu vg bi pu vg 338 bl pu vg 337 151 bl pu vg 171 bi pu vg bl pu vg 24 29 bl pu vg +++ bl pu vg ++vg bl pu+ + pu vg bl ++ + pu+ bl+vg D of that you downloaded in
Linkage Mapping Using a Trihybrid Testcross in Fruit Flies Remember the black, purple, and vestigial genes from Exercise 1? Now you're going to have to construct a map of the same region from trihybrid testcross data. The new map should be similar to the one from Exercise 1, but it won't be identical because we're starting with a new, independent data set. BLACK PURPLE VESTIGIAL TRIHYBRID TESTCROSS In the parental generation, you mate a pure-breeding wild-type female (bl*/bl*.pu*/pu vg+/vg+t with a pure-breeding black, purple, vestigial male (bl/bl pu/pu vg/vg) to produce an F1 generation that is all wild-type (bl*/bl pu*/pu.vg+/vg). Note that the F1 flies are all trihybrid. Next, you mate several F1 trihybrid females (blt/bl:put/pu vg+/vg) with tester males, which are black, purple, vestigial (bl/bl pu/pu.vg/vg). The offspring of this trihybrid testcross are: Phenotype Wild-type Black, purple, vestigial Vestigial Black, purple Purple, vestigial Black Purple Black, vestigial Genotype +/bl;+/pu;+/vg bl/bl;pu/pu;vg/vg +/bl;+/pu;vg/vg bl/bl:pu/pu;+/vg +/bl;pu/pu;vg/vg bl/bl:+/pu;+/vg +/bl; pu/pu;+/vg bl/bl;+/pu;vg/vg Tester Gamete Trihybrid Gamete Number bl pu vg 2332 2333 bl pu vg bi pu vg 338 bl pu vg 337 151 bl pu vg 171 bi pu vg bl pu vg 24 29 bl pu vg +++ bl pu vg ++vg bl pu+ + pu vg bl ++ + pu+ bl+vg D of that you downloaded in
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter6: Cytogenetics: Karyotypes And Chromosome Aberrations
Section: Chapter Questions
Problem 1QP: Analyzing Karyotypes 1. Originally, karyotypic analysis relied only on size and centromere placement...
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