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Name: List the steps in the correct order of DNA mismatch repair in E. coliG
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Description: List the steps in the correct order of DNA mismatch repair in E. coliGroup of answer choicesa. Mut S; Mut H; Mut L; Mut H cleaves unmethylated new DNA strandb. Mut H; Mut L; Mut S; Mut H cleaves unmethylated nascent DNA strandc. Mut H; Mut S; Mut L;

Science

Biology

List the steps in the correct order of DNA mismatch repair in E. coli

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter10: Biotechnology

Section: Chapter Questions

Problem 2RQ: n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules...

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List the steps in correct order of DNA mismatch repair in E. coli Group of answer choices Mut H; Mut L; Mut S; Mut H cleaves unmethylated nascent DNA strand MutH; MutS; Mut L; Mut H cleaves unmethylated nascent DNA strand MutL; MutH; MutS; Mut H cleaves unmethylated nascent DNA strand Mut S; Mut H; Mut L; Mut H cleaves unmethylated new DNA strand
Which of the following DNA repair pathways repair DNA double strand breakage that only occur in diving cells?. Please select all correct answers. A. Nucleotide excision repair (NER) B. Mismatch repair (MMR) C. Double- strand break repair(DSBR) D.non- homologous end joining (NHEJ) E. Synthesis-dependent strand annealing(SDSA)
How would nucleotide excision repair be affected if one of the followingproteins was missing? Describe the condition of the DNAif the repair was attempted in the absence of the protein.A. UvrAB. UvrCC. UvrDD. DNA polymerase
Which of the following types of DNA damage would be hardest to repair using the DNA repair pathways?A. Complete removal of three nucleotides in the middle of one strand.B. A covalent bond between a base on one strand and a base on the complementary strand.C. Incorporation of a sugar other than deoxyribose into one strand.D. Covalent attachment of a short polypeptide to a single base.E. A covalent bond between a base and a deoxyribose on the same strand. Please explain why it's B
All of the following proteins function during nucleotide excision repair, EXCEPT: A. DNA pol B. Uvr A C. Uvr C D. Ligase E. Gyrase
Which of the following repair mechanisms would most likely be used to repair a G in DNA damaged by oxidation? base excision DNA pol I proofreading mismatch repair nucleotide excision
The wrong nucleotide is occasionally incorporated during DNA replication and not immediately repaired by the proofreading function of DNA polymerase. Which of the following DNA repair systems relies upon methylated DNA to determine which base is correct and which needs to be repaired?a) CPD photolyase repair b) mismatch repairc) nucleotide-excision repair d) base-excision repaire) homology-directed repair
Writing a Full Strand:1. A. Original DNA: CCT ATA TCT CTC TAT ATC TCT CAT ACT GTG TGT CTC TAT Complementary DNA:_______________________________________________________________________ B. Make identical strands of DNA CCT ATA TCT CTC TAT ATC TCT CAT ACT GTG TGT CTC TAT (original) ______________________________________________________________________ (new) _____________________________________________________________________ (new) ______________________________________________________________________ (complementary from 1A)2. A. Original DNA: CCG GAT TTT AAT TAG CTA CTA TCG TAC TAC GTT GGT GCT Complementary DNA: _______________________________________________________________________ B. Make identical strands of DNA CCG GAT TTT AAT TAG CTA CTA TCG TAC TAC GTT GGT GCT (original) _______________________________________________________________________ (new) ________________________________________________________________________ (new)…
This is the original strand of DNA: ATG AAG TTT GGC TAA Which option would represent a frameshift mutation due to deletion during replication? ATG UAA GTT TGG CTA A ATG TTT GGC TAA ATG AAG TTT GGC GCA TAA ATG AAG TTG GCT AA
Match the bold DNA repair response(s) to the triggering type of DNA damage. Homologous Recombination Mishmatch Repair Base Excision Repair Nucleotide Excision Repair Non-homologous End Joining Single-strand DNA breaks Removal of repair lesions such as photoproducts caused by UV including Thymine dimers Double-Strand Break repair mechanism which is an accurate repair mechanism without any introduction of insertions or deletions. It requires a sister chromatid as a template. This repair mechanism uses just DNA glycosylase to remove Uracil (no other enzymes or complexes are required) then DNA polymerase can use the template stand to add the complementary base where the Uracil has been removedInterstrand Crosslink Repair This repair mechanism is used to recognize and repair mis-incorporation of base that can arise during DNA replication. Removal and replacement of modifying bases such as Uracil, 8-hyroxyguanine and others. Double-strand Break that is termed as “Quick and Dirty” as it is…
Place the following steps of DNA replication and repair in the correct order by numbering them from 1 to 5. a. A template strand begins to be replicated. b. If the incorrect base is not identiﬁed and replaced, it remains as a point mutation in the DNA. c. DNA polymerase identiﬁes and replaces most incorrect bases with the correct base, complementary to the base on the template strand. d. An incorrect base is added to the growing strand of DNA. e. Proteins identify and replace any incorrect bases missed by DNA polymerase.
Which of the following repair pathways produces more mutations during the process of repair? Group of answer choices DNA polymerase IV None of the above All of the above MutSLH DNA polymerase III UvrABC