ACTIVITY 5.2.3 Make a graphical representation of activation energy and change of free energy in a reaction and compare it with a graph showing the work of enzyme in lowering the activation energy of a reaction.
Q: What type of linkage/s are present in Lactose? a(1 → 4) only O B(1→4) only O a, B(1→ 2) only O a (1→...
A: Lactose is known also as milk sugar since it is found in milk of mammals. It is important as an inf...
Q: Monosaccharide units are hydrophilic and generally soluble in water. What is meant by the statement?...
A: Monosaccharides are carbohydrates that cannot be broken down further by hydrolysis into simpler or s...
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Q: These sugars have at least one common type of linkage EXCEPT maltose lactose sucrose cellobiose
A: Maltose, sucrose, and lactose are examples of disaccharides. Cellulose is an example of a linear pol...
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A: Lysozyme is a low molecular weight enzyme (~14kDa), it hydrolyzed the beta-1,4 linkage between N-ac...
Q: Give the systematic names and the first three digits in the E.C. classifications of the enzymes cata...
A: Enzymes are biocatalyst. They increase the speed of reaction by lowering the activation energy. A fo...
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A: Protein Purification: Modern protein science lab and related industries overexpress target protein i...
Q: Cite one possibility of failure for the glycolysis to proceed.
A: Glycolysis is a cytoplasmic mechanism that creates energy by breaking glucose down into two three-ca...
Q: Chymotrypsin cleaves the N-terminus side of Phe, Tyr, and Trp. O True O False
A: Q20) Part3. Chymotrypsin is a globular protein that acts as a proteolytic enzyme in the digestion sy...
Q: Rank the degree of sweetness of the sugar solutions. 1 – most sweet; 10 – least sweet
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Q: Give a clear handwritten answer of this in which who have long chain of atoms in which molecules con...
A: Introduction: Macromolecules are larger molecules made from smaller ones. These macromolecules are c...
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A: A basic Helix-Loop-Helix Domain (bHLH) is a very common domain found in proteins that act as Transcr...
Q: 3. The rates of a carbonic anhydrase catalyzed reaction of CO2 and H2O, in the absence and presence ...
A: Carbonic anhydrases catalyze the interconversion between CO2 and H2O, maintain acid-base homeostasis...
Q: What factors affect denaturation and refolding of proteins
A: Denaturation(unfolding) is the process in which protein loss the three dimensional structure by brea...
Q: Which peptide will yield the following qualitative results? Fohl's Test (+) Sakaguchi Test (+) Biure...
A: Various tests can be performed on peptides in order to judge the amino acid composition. This is a c...
Q: List some important types of molecular interactions involved in recognition and binding of polysacch...
A: Polysaccharides, also called as glycan are the abundant carbohydrates found in food. They are compos...
Q: Summarize the early evidence indicating that some genes specify the structure of proteins.
A: Proteins have amazing chemical capabilities because the chemical groups on their surfaces frequently...
Q: Look at the structure shown below and answer the following questions: HN NH но NH Download image. a....
A: Peptides or proteins are composed of twenty standard amino acids attached together via peptide bonds...
Q: Which of the following are components of glycogen? glucose and galactose O glucose and fructose gluc...
A: Monosaccharides are the simplest carbohydrate units. These can contain any number of carbons. Howeve...
Q: Enzymes that catalyze DNA denaturation or melting are: gyrases topoisomerases helicases ...
A: DNA is a genetic material that is a double stranded structure polymer of nucleotides. DNA replicatio...
Q: A ligand binds two different receptors with a Kd value of 10−7 M for receptor 1 and a Kd value of 10...
A: Introduction: A protein binds a ligand through a specific reversible reaction. The ligand-binding ca...
Q: Jhat is the correct systematic name for the disaccharide below? (you do not need to know the monomer...
A:
Q: In Figure 8-15, what do you think would be the effect ofa G to A mutation in the first G residue of ...
A: Introduction: The term mutation refers to heritable changes in the genetic material and the individ...
Q: at is the purpose of the PCR technique in Covid testing?
A: The question is all about the PCR technique i.e polymerase chain reaction technique. It is the techn...
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A: Introduction: Polymerase chain reaction involves the ampliifcation of a particular piece of DNA to p...
Q: Predict the results you would get if your Cytosine DNA sample was inadvertently contaminated with th...
A: DNA is a nucleic acids and polymer of nucleotides linked by phosphodiester bonds. It is composed of ...
Q: Which of the following characteristics of B-sheet is incorrect? O In anti-parallel fashion, strands ...
A: Proteins are the polymers of twenty standard amino acids attached together via peptide bonds. The li...
Q: QUESTION 6 Determine the different number of forms that can exist for a ketopentose. 4 16 8.
A: Carbohydrates are composed of carbon, oxygen, and hydrogen which are connected by the gly...
Q: 2. Imagine that you have given blood, but a sample of your red blood cells is accidentally stored in...
A: BLOOD : Blood is a circulating fluid which contains numerous cells , oxygen and suspended in plasma...
Q: 6-One method to determine proximity of two locations in a protein molecule or protein complex is FRE...
A: The principle mechanism of Florescence resonance energy transfer (FRET) is based on the transfer of ...
Q: Why is Covid PCR testing also referred to as reverse transcriptase? O because it is done real-time O...
A: PCR (Polymerase Chain Reaction) is a technique, which is used to amplify a small fragment of DNA. Th...
Q: Which of the following differentiates amylose with cellulose? The two polysaccharide have different ...
A: Amylose : It is a storage polysaccharide Cellulose : It is a structural polysaccharide
Q: Which tests both result in a colorimetric change due to furfural complexation? Molisch's and Benedi...
A: Molisch's test detects the presence of carbohydrates in a given sample. It distinguishes carbohydrat...
Q: Having the ability to degrade the DNA allows the DNA polymerase to perform its job properly and effi...
A: DNA replication is a complex process of producing copies of the entire chromosome using the templa...
Q: At high salt concentration, proteins are less soluble. Changing the solubility of a protein based on...
A: Salting out procedure can be used to precipitate large biomolecules, such as proteins or DNA. The sa...
Q: .Linoleic acid and linoleic acid cannot be synthesized in human tissues, because of _____. * a.Hum...
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Q: What amino acids are present on the unknown sample based on the results below? Test/s Results Ninhyd...
A: There are several test, which are used to perform in order to identify the specific amino acid in a ...
Q: QUESTION 7 The monosaccharides shown below are H. HO H -HO- HO H H- HO H но HO- HO- CH,OH CH,OH O En...
A: Isomers are molecules with the same molecular formula, but different arrangement of atoms. Isomers a...
Q: The enzymatic activity of an enzyme with Kg = 2 mM that converts substrate S into product P is measu...
A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that o...
Q: n the insulin signaling pathway, the function of the PIP3-dependent Protein Kinase-1 is to add a __ ...
A:
Q: n the living cell, free energy made from one reaction can be used to drive another in an energetical...
A: Glycolysis is also known as the Embden Meyerhof pathway and it is highly conserved from ...
Q: Which of the following statements concerning carbohydrates is correct? O They can exist in left-hand...
A: Carbohydrates are very important biochemical molecules of a human body but certainly not the most ab...
Q: When the enzyme is incubated with oxaloacetate, will oxaloacetate be observed?
A: Many amino acid biosynthetic pathways involve transamination reactions. The α-amino group from one ...
Q: With the aid of the simple generic diagram, identify and explain how the type of chemical bonding st...
A: An alpha helix is a periodic secondary structure in proteins. This secondary structure is stabilized...
Q: Draw the orbital diagrams of the following elements: Silicon Охудen Chromium
A: Introduction: Orbital diagram help visualize which orbitals the electrons in an atom are. There are ...
Q: Substrate, uM VO, uM/min 2.5 28 4 40 10 70 20 95 40 112 100 128 200 139 1000 140
A: The Lineweaver Burk (LB) equation is; 1V0=KmVmax×[S0] +1Vmax This is of the form 'y=mx+c' Hence i...
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- 12. Explain why those biological reactions that have their equilibria shifted towards theproducts have negative values for ΔGo of reactions. Explain how equilibria relates toGibbs free energy.Describe the position of the transition state on a vertical energy scale, from low to high, relative to the position of the reactants and products, for both endergonic and exergonic reactions.The energy released by the hydrolysis of ATP is primarily stored between the alpha and beta phosphates equal to -57 kcal/mol harnessed as heat energy by the cell to perform work providing energy to coupled reactions
- Trend observed in graph and conclusion about the effect of temperature on enzyme activity. i) include a concise description of the trend observed in the graph shown in question 3 above, and explain this trend using the language presented in this unit and your biochemical knowledge of enzymes and reactions. In your conclusion, provide a logical argument supported by molecular theory that would explain any change observed in enzyme activity.Initial rate data for an enzyme that obeys Michaelis–Menten kinetics areshown in the following table. When the enzyme concentration is 3 nmolml-1, a Lineweaver–Burk plot of this data gives a line with a y-intercept of0.00426 (μmol-1 ml s). (a) Calculate kcat for the reaction.(b) Calculate KM for the enzyme.(c) When the reactions in part (b) are repeated in the presence of 12 μM ofan uncompetitive inhibitor, the y-intercept of the Lineweaver–Burk plotis 0.352 (μmol-1 ml s). Calculate K′I for this inhibitor.22. What is a major benefit of calculating catalytic efficiencies? Group of answer choices It determines whether a catalytic reaction is cooperative It allows you to compare different substrate preferences for an enzyme It tells you the rate of an enzyme reaction It allows you to determine the efficiency of the enzyme being studied
- Mechanisms of catalysis: 2.5 Mechanism of chymotrypsin summary. These mechanisms involve several of the above-mentioned catalyses. In these summaries, do not just draw a diagram of the proposed mechanisms. It is more important to understand which reaction steps involve what kind of catalysis and how these help to reduce the activation energy needed for the reaction (e.g. a step in the reaction mechanism could be electrostatic catalysis to stabilise the transitions state)Activation energy (a) Draw a general energy diagram for a chemical reactionproceeding from reactants to products. (b) Indicate on your plot the Transition State withX-double dagger. (c) Add the energy profile for the reaction as catalyzed by an enzyme.(d) Indicate the energy of interactions with the enzyme that stabilize the transition state.22. In Exergonic reaction, all of the following are true except A. Spontaneous reaction B. Delta G is positive C. free energy of substrate is more than that of products D. catabolic reaction
- An enzyme contains an active site aspartic acid with a pKa = 5.0, whichacts as a general acid catalyst. On the accompanying template, draw thecurve of enzyme activity (reaction rate) versus pH for the enzyme (assumethat the protein is stably folded between pH 2–12 and that the active siteAsp is the only ionizable residue involved in catalysis). Briefly explain theshape of your curve.Reaction 25.2 Glucose (aq) + PO43- (aq) ⇄ Glucose-PO43- (aq) Reaction 25.2 is nonspontaneous and very slow. Use this information to sketch a reaction diagram for Reaction 25.2. Clearly label the x axis, y axis, products, reactants, and activation energy.Select true if the statement is CORRECT and false if OTHERWISE 1. Enzymes are catalysts and increase the speed of a chemical reaction without themselves undergoing any permanent chemical change. 2. Catalysis is defined as the acceleration of a chemical reaction 3. if the amount of the enzyme is kept constant and the substrate concentration is then gradually increased, the reaction velocity will decrease. 4. In the Induced-fit Model, if a dissimilar substance which does not fit the site is present, the enzyme rejects it 5. The Michaelis constant Vo is defined as the substrate concentration at 1/2 the maximum velocity. 6. A prosthetic group - an organic substance which is dialyzable and thermostable which is firmly attached to the protein or apoenzyme portion. 7. The rate of an enzyme-catalyzed reaction increases as the temperature is raised beyond optimum temperature. 8. Enzymes can be classified by the kind of chemical reaction catalyzed. 9. The living cell is the site of tremendous…
