Micropipettes have a two-position plunger with friction stops.  Depressing to the (first / second) stop measures the desired volume and is the one you should use to fill the tip. The ( first / second) stop is used to introduce an additional volume of air to blow out any solution remaining in the tip when you are emptying the tip.

Basic Clinical Lab Competencies for Respiratory Care: An Integrated Approach
5th Edition
ISBN:9781285244662
Author:White
Publisher:White
Chapter28: Weaning And Discontinuation Of Mechanical Ventilation
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Micropipettes have a two-position plunger with friction stops. 

  1. Depressing to the (first / second) stop measures the desired volume and is the one you should use to fill the tip.
  2. The ( first / second) stop is used to introduce an additional volume of air to blow out any solution remaining in the tip when you are emptying the tip.
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