n 1- Question 14 ation of cells and cellular components is accomplished with a centrifuge. A sample of cells is first blended to mechanically break apart some of the cells releasing their contents to form a cell te. The remaining whole cells and cell components are separated based on their sedimentation rate by centrifuging the sample at increasingly high speed. The centrifuge spins at up to several rotations per minute (rpm) forcing material to accumulate as a pellet at the bottom of the tube. The liquid portion is removed and centrifuged again to yield four (4) pellets containing the cells or cell ts listed in the diagram. Low speed wate 1 2 3 = Medium speed Pellet 1 Whole cells Nuclei Cytoskeletons 1 .. High speed ration of whole cells is required for the diagnosis and treatment of certain disease processes in humans. Four patients each exhibiting unique symptoms are shown in the table. The symptoms of ent can be explained based on damage to one, and only one, organelle or cell structure. Pellet 2 Mitochondria Lysosomes Peroxisomes Very high speed Pellet 3 Microsomes Other small vesicles Pellet 4 Ribosomes Viruses Large macromolecules Some of the symptoms In the patients Body aches (Fabry disease), due to accumulation of proteins and fat in cells. Lack of alpha-galactosidase enzyme and other digestive enzymes. Muscle fiber weakness; exercise intolerance, multiple organ failure. Incomplete fingernails due to low keratin protein; un-pigmented spots on skin, due to low melanin protein production (DKC disease). Damaged cell function and shape due to loss of desmin and other filaments network. TWO options would doctors expect to find evidence explaining the symptoms experienced by each patient? Pellets 1 and 3 because it's likely that the patients have damage in their cytoskeleton, microsomes, and other small vesicles. Pellets 1, 2 and 3 because it's likely that the patients have damage in their cytoskeleton, mitochondria, and microsomes. Pellets 1, 2 and 4 because it's likely that the patients have damage in their cytoskeleton, peroxisomes, and ribosomes. Pellets 2 and 3 because it's likely that the patients have damage in their mitochondria, lysosomes, and microsomes. Pellets 2 and 4 because it's likely that the patientshave damage in their lysosomes, mitochondria, and ribosomes.

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Section 1- Question 14
The separation of cells and cellular components is accomplished with a centrifuge. A sample of cells is first blended to mechanically break apart some of the cells releasing their contents to form a cell
homogenate. The remaining whole cells and cell components are separated based on their sedimentation rate by centrifuging the sample at increasingly high speed. The centrifuge spins at up to several
thousand rotations per minute (rpm) forcing material to accumulate as a pellet at the bottom of the tube. The liquid portion is removed and centrifuged again to yield four (4) pellets containing the cells or cell
components listed in the diagram.
Cell
homogenate
Patient
Patient 1
Patient 2
Patient 3
Low speed
Patient 4
Medium speed .. High speed
..
-
☐☐☐☐
Pellet 1
Whole cells
Nuclei
Cytoskeletons
The separation of whole cells is required for the diagnosis and treatment of certain disease processes in humans. Four patients each exhibiting unique symptoms are shown in the table. The symptoms of
each patient can be explained based on damage to one, and only one, organelle or cell structure.
Pellet 2
Mitochondria
Lysosomes.
Peroxisomes
..Very high speed
1
Pellet 3
Microsomes
Other small
vesicles
↓
Pellet 4
Ribosomes
Viruses
Large macromolecules
Some of the symptoms In the patients
Body aches (Fabry disease), due to accumulation of proteins and
fat in cells. Lack of alpha-galactosidase enzyme and other
digestive enzymes.
Muscle fiber weakness; exercise intolerance, multiple organ
failure.
Incomplete fingernails due to low keratin protein; un-pigmented
spots on skin, due to low melanin protein production (DKC
disease).
Damaged cell function and shape due to loss of desmin and other
filaments network.
In which TWO options would doctors expect to find evidence explaining the symptoms experienced by each patient?
A Pellets 1 and 3 because it's likely that the patients have damage in their cytoskeleton, microsomes, and other small vesicles.
B Pellets 1, 2 and 3 because it's likely that the patients have damage in their cytoskeleton, mitochondria, and microsomes.
C Pellets 1, 2 and 4 because it's likely that the patients have damage in their cytoskeleton, peroxisomes, and ribosomes.
D Pellets 2 and 3 because it's likely that the patients have damage in their mitochondria, lysosomes, and microsomes.
E Pellets 2 and 4 because it's likely that the patientshave damage in their lysosomes, mitochondria, and ribosomes.
Transcribed Image Text:Section 1- Question 14 The separation of cells and cellular components is accomplished with a centrifuge. A sample of cells is first blended to mechanically break apart some of the cells releasing their contents to form a cell homogenate. The remaining whole cells and cell components are separated based on their sedimentation rate by centrifuging the sample at increasingly high speed. The centrifuge spins at up to several thousand rotations per minute (rpm) forcing material to accumulate as a pellet at the bottom of the tube. The liquid portion is removed and centrifuged again to yield four (4) pellets containing the cells or cell components listed in the diagram. Cell homogenate Patient Patient 1 Patient 2 Patient 3 Low speed Patient 4 Medium speed .. High speed .. - ☐☐☐☐ Pellet 1 Whole cells Nuclei Cytoskeletons The separation of whole cells is required for the diagnosis and treatment of certain disease processes in humans. Four patients each exhibiting unique symptoms are shown in the table. The symptoms of each patient can be explained based on damage to one, and only one, organelle or cell structure. Pellet 2 Mitochondria Lysosomes. Peroxisomes ..Very high speed 1 Pellet 3 Microsomes Other small vesicles ↓ Pellet 4 Ribosomes Viruses Large macromolecules Some of the symptoms In the patients Body aches (Fabry disease), due to accumulation of proteins and fat in cells. Lack of alpha-galactosidase enzyme and other digestive enzymes. Muscle fiber weakness; exercise intolerance, multiple organ failure. Incomplete fingernails due to low keratin protein; un-pigmented spots on skin, due to low melanin protein production (DKC disease). Damaged cell function and shape due to loss of desmin and other filaments network. In which TWO options would doctors expect to find evidence explaining the symptoms experienced by each patient? A Pellets 1 and 3 because it's likely that the patients have damage in their cytoskeleton, microsomes, and other small vesicles. B Pellets 1, 2 and 3 because it's likely that the patients have damage in their cytoskeleton, mitochondria, and microsomes. C Pellets 1, 2 and 4 because it's likely that the patients have damage in their cytoskeleton, peroxisomes, and ribosomes. D Pellets 2 and 3 because it's likely that the patients have damage in their mitochondria, lysosomes, and microsomes. E Pellets 2 and 4 because it's likely that the patientshave damage in their lysosomes, mitochondria, and ribosomes.
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