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- Calculate the concentration of the ligand required to fill 25% of the binding site. X+A ⇌ XA , ka = 1.5 ×10-6 Y+A ⇌ YA , ka = 4.7 × 10-4 (i.e, q = 0.25) for both proteins.Explain the importance of Stemoamide ?1. AsnIleGlyTyrGluProSerArgAsp 2. MetPheAsnSerGluProLysCys 3. SerArgGluTyrValValThrPheGly 4. MetPheSerThrAspGluVal