a) Which strand is coding strand? b) What is the sequence of the encoded polypeptide? A mutant gene has GC (bold) to TA substitution @ position 20. c) What is the sequence of the mutant polypeptide d) What effect is the mutation likely to have on function of the protein? Explain with reasoning.
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
- The DNA sequence below is from the center of a protein coding region.
5 10 15 20 25 30
5’ …… TATCC TAGAG CATAA TTTCG AGATA GCTAG …… 3’
3’ …… ATAGG ATCTC GTATT AAAGC TCTAT CGATC …… 5’
a) Which strand is coding strand?
b) What is the sequence of the encoded polypeptide?
A mutant gene has GC (bold) to TA substitution @ position 20.
c) What is the sequence of the mutant polypeptide
d) What effect is the mutation likely to have on function of the protein? Explain with reasoning.
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