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list the stains and decolorizing agent used in acid fast staining method.
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- Differentiate the “hot method” and “cold method” of acid-fast stainingWhy must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle, procedure and results.
- What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with purple color. Describe the organism with pinkish red color.Types of Differential Staining techniques. 1. Gram stain 2. Acid-fast stain Please explain, thank you.What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?
- Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of simple staining and gram staining. 1. Simple StainingWhat is the difference between simple and differential stains? Give an example of a differential stain.Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?