Please describe the pros and cons of the following methods for counting bacterial cells. 1) Petroff-Hausser counting chamber 2) Coulter Counter 3) Plate Count
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Please describe the pros and cons of the following methods for counting bacterial cells.
1) Petroff-Hausser counting chamber
2) Coulter Counter
3) Plate Count
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- Describe three problems associated with using the standard plate count method for determining the number of bacteria in a sample.What are the disadvantages of using the mouth in filling a pipette? Why do we use exponential notation in counting bacteria? Why is serial dilution necessary?In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?
- Discuss the application of the following techniques in histopathology: 1.2.1. Immunohistochemistry 1.2.2. Fluorescent in situ hybridization 1.2.3. Nucleic acid amplificationHow do we reconcile the number of colonies in the plates to the actual number of bacteria in the original sample? Count Plate MethodHow can colony morphology be used in the identification of bacterial species? What are the environmental factors that affect the growth of bacteria? What is agar (or agar-agar)? Is this different from nutrient agar? Why? How is xylol used to clean the lens of a microscope? What may happen if xylol is constantly used for this purpose? For what purpose would you adjust each of the following microscope components during a microscopy exercise? iris diaphragm coarse adjustment knob fine adjustment knob condenser mechanical stage control
- What is the difference between culture-based technique and culture-independent techniques based on the principle behind the method?What are the different manufacturing methods of Suppositories dosage form? Explain heat molding process using flow chart. Please explain at your own words.What order should the steps be in for this culture method?