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- With the discovery of CHIP28 (aquaporins), do you think water can move across a membrane even if the cell is dead? Explain. Thank you for the help!The CMC of SDS is 8.2 mM at 25 °C. Please describe the biological membrane solubilization when [SDS] is below or above 8.2 mM in the solution.Isotonicity. Show your complete solution. 1. A hospital pharmacist fills a medication order calling for an intravenous fluid of dextrose 5% in a 0.9% sodium chloride injection and 40 mEq of potassium chloride in a total volume of 1000 mL. The intravenous infusion is administered through an IV set that delivers 15 drops per milliliter. The infusion has been running at a rate of 12 drops per minute for 15 hours. During the 15-hour period: (a) How many mEq of KCl have been administered? (b) How many grams of KCl have been administered? (c) How many millimoles of KCl have been administered? (d) What is the total osmolarity of the intravenous fluid? Express the answer in millimoles (rounded to the nearest whole number) per 1000mL.
- 3 cell membrane samples: A, B, and C were fluorescently labeled and subsequently photobleached. The recovery of fluorescence is given on the FRAP curve: (see image attached) Rank the 3 cell membranes in terms of DECREASING fluidity and explain your ranking based on: their FRAP curves; most probable appearance; and most probable composition of the membranesDemonstration of Osmosis using Potato Osmometer 1. What will happen when the liquid in the cavity overflows? ExplainElectrochemical gradients (a) power simple diffusion (b) are established by pinocytosis (c) are necessary for transport by aquaporins (d) are established by concentration gradients (e) are a result of both an electric charge difference and a concentration difference between the two sides of the membrane
- Diffusion experiments of a small molecule drug in tissue samples are performed. The drug has a radius of 2.24 nm and a pure-water diffusivity of 2.75 x 10^-6 cm^2/s. Cells occupy approximately 72% of the tissue byvolume. The molecule is very hydrophilic and does not readily cross cellular membranes. If you assume that the interstitial space is gel-like with a microstructure parameter k=3.7 x 10^-3 nm^-1, what is the effective diffusivity in the tissue?xplain how the following affect membrane fluidity:– Level of phospholipid tail saturation– Level of cholesterol– Phospholipid tail length• Estimation of the Water Potential (Ψw) of Storage Tissue by Volume Change Method Observe these experiments in these links: https://www.youtube.com/watch?v=jTDATlaBV-o https://www.youtube.com/watch?v=k1O9jBHgsxs Estimates of water potential of various tissues have been obtained after equilibrating the tissues in solutions of different osmotic potentials. The principle depends upon finding the concentration of sucrose that would cause no change in weight of the tissue before and after an incubation period in the solution. This concentration may be assumed to have a water potential equal to that of the tissue.
- Quantitative – diffusion time: Consider a spherical, single-celled organism which needs oxygen tosurvive. a. Assume oxygen needs to diffuse from the outside of the cell to its center. Write an equation thatgives the diffusion time in terms of the cell radius. b. The cell has a 4 micron (m) diameter, and diffusion coefficient of oxygen is 1.8 x 10-5 cm2/s inwater (assume diffusion is the same through the cell membrane). How long does it take for oxygento diffuse to the center of the cell? c. Cells typically need key molecules such as oxygen to survive. Diffusion times of oxygen can limithow big cells can become. If the cell gets too large for oxygen to reach its center, the cell coulddie. Calculate the size of a cell at which oxygen diffusion time becomes too slow and so limits theability of the cell to get that big. Explain your assumptions. How does this compare to typical cellsizes?Chemistry Q4 1mL of the stock solution acetaminophen (1mg/mL) is used for the estimation of PC using n-octanol. After extraction, 0.1mL of the extracted solution was added to 0.9mL of water and 100uL of this solution was loaded in 96 well plate to the absorbance at 260nm. If the observed absorbance of the extraction phase is 0.2 using the following standard curve equation, Calculate the concentration of acetaminophen in aqueous Phase. Note: If you need the standard curve equation, please make It up because I want to understand the concept. Please explain in steps like how to approach the problemDesign an experiment to study the effect of solvent on the integrity of the membrahe of carrot-root cells. Remember that carrot contains carotenoids which include the yellow-orange colored pigments which can be collectively measured by a spectrophotometer at 450 nm. Also remember that these group are fat soluble. State your hypothesis, a brief summary of the experimental procedure and conditions, the expected outcome(s) and conclusion(s)