1mL of the stock solution acetaminophen (1mg/mL) is used for the estimation of PC using n-octanol. After extraction, 0.1mL of the extracted solution was added to 0.9mL of water and 100uL of this solution was loaded in 96 well plate to the absorbance at 260nm. If the observed absorbance of the extraction phase is 0.2 using the following standard curve equation, Calculate the concentration of acetaminophen in aqueous Phase. Note: If you need the standard curve equation, please make It up because I want to understand the concept.

1mL of the stock solution acetaminophen (1mg/mL) is used for the estimation of PC using n-octanol. After extraction, 0.1mL of the extracted solution was added to 0.9mL of water and 100uL of this solution was loaded in 96 well plate to the absorbance at 260nm. If the observed absorbance of the extraction phase is 0.2 using the following standard curve equation, Calculate the concentration of acetaminophen in aqueous Phase. Note: If you need the standard curve equation, please make It up because I want to understand the concept.

Biochemistry

9th Edition

ISBN:9781305961135

Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Chapter15: The Importance Of Energy Changes And Electron Transfer In Metabolism

Section: Chapter Questions

Problem 12RE: MATHEMATICAL Consider the reaction AB+C, where G=0.00. (a) What is the value of G (not G) when the...

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Q4 1mL of the stock solution acetaminophen (1mg/mL) is used for the estimation of PC using n-octanol. After extraction, 0.1mL of the extracted solution was added to 0.9mL of water and 100uL of this solution was loaded in 96 well plate to the absorbance at 260nm. If the observed absorbance of the extraction phase is 0.2 using the following standard curve equation, Calculate the concentration of acetaminophen in aqueous Phase.

Note: If you need the standard curve equation, please make It up because I want to understand the concept.

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