Place the compounds in the order they will elute from the column when separated using reverse-phase liquid chromatography (RPLC). CH3 CH3 p-xylene napthalene CH3 anisole OH phenol anisole p-xylene Elutes first Elutes last Answer Bank naphthalene phenol
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- Indicate whether TLC (thin-layer chromatography) or column chromatography is more appropriate for the following:a. This is an analytical method for separating compounds of amixture.b. The solvent front moves downward.c. This is a preparative method for separating a mixture ofcompounds.What would happen if a mobile phase of ethyl acetate/triethly amine is used in column chromatography for (3-nitrotoluic acid, 2-naphthol and 1,4-dimethoxybenzene)Which of the solvents below can be used with reversed phase chromatography? a. Acetonitrile b. Toluene c. Hexane d. Carbon tetrachloride
- Identify the detectors in gas chromatography that can be used for the detection of each type of analyte. More than one detector may be appropriate for a given analyte, and a given detector may be appropriate for more than one type of analyte.To separate a mixture of compounds containing the following two compounds column chromatography was used by a student. Stationary phase was silica gel and mobile phase was 95 % ethyl acetate and 5 % Hexane mixture. Which compound elutes first through the column? WhyUpon screening with TLC (made of silica gel) chromatography using hexane/chloroform (3:1) as eluting solution, 3 spotswere detected corresponding to a (A) polyphenol, a (B) tannin and an (C) alkaloid. Write the letters of the answer onthe spots in the chromatogram shown at the picture.
- The compounds biphenyl and naphthalene are presented in a mixture. Can hexane be used as a solvent to separate the compounds by column chromatography with silica gel as the stationary phase? Why or why not.Based on the resulting chromatograms after visualization with iodine vapor, which between the solvent systems WHITE and MIX resulted in a more effective separation of the pigments of the indigo extract? Explain your answer in terms of the solvent that did not effectively separate the components of the root extract.Iman has a mixture of two compounds A and B. A is a protein, and B is a lipid. He plans to separate the mixture using a column chromatography technique. He packed the column with amino bonded with siloxane material and use a mixture of 20:80 of water: hexane as the mobile phase. i) Sketch the apparatus set up for the chromatographic technique used by Iman. Use the correct chromatographic terminologies to label all of the apparatus, materials, and phases. ii) Predict the sequence of elution when the mixture was loaded onto the separation column. Justify your answer. iii) After the first compound was eluted, Iman discovered that the second compound was taking too long to pass through the column. Please suggest any strategies that could be used to aid the elution process.
- Gestation time arrangement of Hegran, Hexanol, and Benzene compounds in the reverse phase of column liquid chromatography.4) If the classification of the chromatography run is a reverse-phase chromatography, discuss the relative polarities of caffeine, aspartame and benzoate.Using reversed phase chromatography in order to separate chloroquine and hydroxychloroquine. Which will be the first one to elute out of the column? Or both will elute the same time?