1.Promoters usually contain an AT-rich sequence. How is this beneficial to transcription?
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- 1.This is where the RNA polymerase is bound and a short region of DNA is accessible to begin the process of transcription. Group of answer choices a. Closed Promoter Complex b. Open Chromatin c. Closed Chromatin d. Open Promoter Complex 2.The nucleotide near the 3' splice site of an intron that joins with a Guanine located at the 5' splice site by a 2'-to5' phosphodiester bond is called the: Group of answer choices a. Branch Point Adenine b. Branch Point Thymine c. Branch Point Uracil d. Branch Point Cytosine 3.The form of chromatin in which histone 1 partially condenses chromatin fibers into a coiled form is called: Group of answer choices a. 10 nm fibers b. Heterochromatin c. 300 nm fibers d. Solenoid 4.Exchange of genetic information between homologous chromosomes. Group of answer choices a. Homologous Recombination b. Gene Flow c. Gene Conversion d. Homologous RepairA certain template DNA strand has the following nucleotide sequence: 3’-TACTCGATGCTGTGCGAT-5’ a) What would be the nucleotide sequence of the complementary nontemplate DNA strand? What is this process called and where does this occur in the eukaryotic cell? b) Take the template strand through the process of transcription. What is the resulting strand called and where does this process occur in a eukaryotic cell. c) Take the template strand through the process of translation including the location in the cell. You should finish with a polypeptide chain. You will need to use the Genetic code found in your notes/bookThe following diagram represents a transcription unit in a hypothetical DNA molecule. 5′ … TTGACA … TATAAT … 3′ 3′ … AACTGT … ATATTA … 5′ a. On the basis of the information given, is this DNA from a bacterium or from a eukaryotic organism? b. If this DNA molecule is transcribed, which strand will be the template strand and which will be the nontemplate strand? c. Where, approximately, will the transcription start site be?
- First, I bind to an unstructured region of RNA and moves toward its 3’ end. Then RNA polymerase encounters a terminator sequence and I am able to catch up. Finally, using helicase activity, I am able to unbind the DNA-RNA hybrid and transcription stops. Who am I? What process do I work in? Group of answer choices Rho, in rho- dependent terminator in eukaryotes Rat1, in rho-independent terminators in prokaryotes Rho, in rho- dependent terminators in bacteria The correct answer is not available Rat1, in termination in eukaryotes1. In DNA amplification, using the Taq polymerase, what is the maximum number of amplification cycles? a. 30 b. 20 c. 60 d. 10 2. In the case of trp operon, for transcription to fully occur, which of the following conditions must be met? a. Environmental tryptophan level must be low. b. Environmental tryptophan must be absent. c. Environmental tryptophan level must be abundant. d. Both a and b are correct.1. What is a consensus sequence? What are the components of RNA polymerase and what are their functions? 2. Describe the steps involved with transcription initiation.? Describe the steps involved with transcription elongation. 3. Describe the steps involved with transcription termination. A consensus sequence is a sequence of DNA,RNA, or protein that has aligned sequence.
- f you made a change in the promoter sequence in the DNA that inactivates the promoter, what would happen at the RNA level? A-Nothing, because the RNA would be made as usual B-Transcription factors would be unable to bind and the RNA polymerase would not be recruited to the DNA, so no RNA would be made. C-The mutation of the DNA would be carried through to the RNA sequence. D-The DNA helicase would not be able to recognize and bind the DNA, so the RNA would not be made. EXPLAIN WHY THE ANSWER YOU CHOOSE IS CORRECT1. Eukaryotic transcription initiation begins at the TATA site approximately 30 bp upstream of the transcription start site. One subunit of TFIID (Transcription Factor II D) binds at the TATA site. What is this subunit? 2. This initial binding at the TATA site is specific and coincides with the binding of the entire TFIID complex. This results in a massive change of shape to the DNA helix of the promoter region. After TFIID binding and this shape change, another GTF(General Transcription Factor) can bind. What is this next GTF? 3. Next, which GTF binds to a DNA sequence in the promoter region and to the TFIID-promoter complex before the binding of the RNA Pol II-TFIIF complex? 4. Describe how would you redraw Figure 13-16 to reflect the change in the dsDNA helical shape due to the initial binding to the TATA site? 5. What other…1. What term is referred to the process of removing large portions of the RNA primary transcript molecules and reconnecting the remaining portions? A. Exon splicing B. UTR splicing C. Intron splicing D. RNA splicing 2. Which of the following does not involved in the proofreading and repair of DNA? A. Isomerase B. Ligase C. DNA Polymerase I D. Nuclease
- The following represents a transcription unit in a hypothetical DNA molecule in E.coli. (Transcription can use either strand, but by convention we always put 5’à 3’ on the top.) 5’…..TTGACANNNNTATAAT…3’ 3’…..AACTGTNNNNATATTA…5’ If this molecule is transcribed from left to right, then the ____ strand will be the template strand and the ___ strand will be the non-template strand. top, bottom bottom, top1. Transcription: a)State the role of RNA polymerase in gene transcription.b. Explain why the DNA is not used directly for protein translation (i.e., why is mRNA used instead?).c. Explain what occurs when a gene’s promoter region is open for RNA polymerase binding.d. Explain what occurs when a gene’s promoter regions is blocked from binding RNA polymerase.e. Explain how two cells, such as liver cells and skin cells, can become specialized in structure and function despite containing the same genome.A binding .site for RNA polymerase is called a ___ . a. gene b. promoter c. codon d. protein
