Prepare 100ml of TBST using 10% Tween 20 and 10x PBS (Final 1x PBS and 0.05% Tween 20). Could you please show and explain the calculations?
Q: Fetal gamma-subunits cause... Question 29 options: Decreased 2,3-bisphosphoglycerate binding a...
A: Red blood cells carry hemoglobin, which is a protein. Hemoglobin absorbs oxygen from the lungs and t...
Q: Draw the following amino acids interactions: 1. H-bond - between -0H of Serine amino acid and C=0 b...
A: A hydrogen bond is formed between a hydrogen atom which is covalently bound to a more electronegativ...
Q: OH HO HO A NH2 Но. HO R-O. HO. HO HO R-O, OH Но HO `OH OH Но R-O, D HO. но но HO Но `OH OH B но. `OH...
A: Glycosidic bond is a type of covalent bond formed after reaction between hydroxyl group of carbohydr...
Q: Is the Salkowski test a specific test for cholesterol? Justify your answer.
A: Cholesterol is a type of steroid found in animals. It is the major component of the cell membrane. ...
Q: For the oligopeptide below Gly-Asp-Ser-Cys-His pKa of terminal amino group: 8.0 pKa of Asp: 4.1 pka ...
A: The given oligopeptide is composed of glycine, aspartic acid, serine, cysteine, and histidine amino ...
Q: 1. What is animal oil/fat used for? 2. Why are animal fat not used in cooking? 3. What is the differ...
A: Since you have posted a question with multiple sub-parts, we will solve first three sub-parts for yo...
Q: 10. What is the energy yield in ATP molecules associated with cach of the following: a. Glucose > 2 ...
A: Hi! Thank you for the question, as per the honor code, we are allowed to answer the first q...
Q: 2. Briefly answer the following questions. A. The primary sequence of an enzyme indicated the presen...
A: A. During the lifetime of a protein there are many points at which an acetyl group may be added to i...
Q: Draw out the peptide DPGS as it would appear in a Type II β-turn and clearly label/identify all the ...
A: Beta turns are a prominent secondary structure seen in proteins. The 2 most common types of beta tur...
Q: Answer the question with the multiple choice. 1. What does the term metabolism refer to? a. The b...
A: Certain terminologies are important in biology, biochemistry and anatomy and physiology. These terms...
Q: What would be the difference in oxygen availability in a beaker versus an inverted test tube when de...
A: The answer of the following question is given below
Q: What is the difference between cellulose and starch? Select one O a Starch is unbranched, while cell...
A: Carbohydrates are composed of carbon, oxygen, and hydrogen which are connected by the gly...
Q: Why can’t Myosin V motor proteins “walk” towards the negative end of the actin molecule? Why do most...
A: Sarcomere is the contractile unit in muscles. The figure below shows a Sarcomere The blue color ind...
Q: The following compound is: O a. A classical synthetic cannabinoid. O b. A non-classical synthetic ca...
A: Cannabinoids are naturally occurring compounds found in the Cannabis sativa plant whereas aminoalkyl...
Q: Kindly answer numbers 1, 2, and 3 please
A: Glycogen is a one of the multibranched polysaccharide of glucose which is serves as a form of energy...
Q: List down the substances in which both specific and optical rotation are needed to be measured. And ...
A: The rotation of the plane of polarisation of linearly polarised light as it travels through specific...
Q: What is oxidative degradation in catabolism? and what is reductive biosynthesis in anabolism?
A: Anabolic reactions lead to the synthesis of biomolecules. The catabolic reactions lead to the oxidat...
Q: Straight hair can be curled and curly hair can be straightened by exposing wet hair to a reducing ag...
A: The innermost part, or the root, of your hair is called a hair follicle. Hair follicles anchor each ...
Q: Which of the following statements best describes the role of the enteric coating on aspirin tablets,...
A: Aspirin, also known as acetylsalicylic acid, is a drug that can be used to relieve pain, minor aches...
Q: What are the complications of hypoglycemia? Hyperglycemia?
A: Glucose is the most abundant sugar in our bodies. It is either directly ingested or generated in the...
Q: Concerning the use of pyruvate when ratio of NADH/NAD+ is low, what is the fate of the carbon labele...
A: The answer of the above question is: D Pyruvate is never metabolised by the cell, instead pyruvate...
Q: The concentration of the synthetic opioid fentanyl should be determined in a blood plasma sample fro...
A: Introduction' Fentanyl is a μ-opioid receptor agonist with strong anesthetic and analgesic propertie...
Q: Below is the set of kinetics data of a velocity of an enzymatic reaction without inhibitor (Vo) and ...
A: Multiple questions asked. I will answer only first question, as allowrd by guidelines. Kindly repo...
Q: BIM-46187 is a protein inhibitor that binds to the a-subunit of the G. protein. It prevents the GDP/...
A: Cell signaling is the conversion of the information in a signal into cellular response. Ligands, rec...
Q: OH но- HO он но но- OH
A: Glycosidic linkage: This is a type of covalent bond joining two sugar molecules. It is formed betwee...
Q: What statement about hemoglobin's integrated transport of oxygen, carbon dioxide and protons is FALS...
A: The incorrect statement from above option is: Proton binding stabilizes the R- form of hemoglobin. E...
Q: 15. Name the peptide он он H H,'N-CH-C-N-CH-C-N-CH-C-0- CH, CH CH-CH, SH ČH, A. Methionyl-leucynyl- ...
A: Peptides are small amino acid chains connected to each other through peptide bonds. Based on the num...
Q: Can you please help find what Gln is at ph 9.5?
A: Amino acids are the smallest part that makes proteins or peptides. There are twenty standard amino a...
Q: 1. Prepare stock solutions of the 3 proteins in 0.8 mM Phosphate buffer pH=7.40 10 μΜ Conc.A 25 µM L...
A: Here Molar Concentration, Molecular weight and Volume of stock solution is given Molar concentration...
Q: SELIWANOFF'S TEST You can use this as your reference : https://youtu.be/rKng5-ij6kQ
A: Sugars are polyhydroxy compounds that have a general formula of CnH2nOn. They either contain an alde...
Q: B-subunit Histidine 146 NH + H ÓH NH, Aspartate 94 H2N Which statement concerning NH2 this salt brid...
A: Hemoglobin is an oxygen transport protein that is found in the cytoplasm of red blood cells. Hemoglo...
Q: A protein that is normally found in an aqueous solution has these amino acids in its primary structu...
A: Proteins are composed of the linear chain of amino acid sequences attached via peptide bonds. Protei...
Q: Derive a mathematical equation that calculates the speed at which DNA molecules move when placed ins...
A: Electrophoresis is a process where DNA is separated on the basis of there molecular size. the term E...
Q: What is the role of chloride in biochemical processes of the body? 2. What are the other test for ...
A: Body has different electrolytes. Chloride being one of the most important electrolytes in the blood
Q: Lactose is made up of D-galactose and one unit of D-glucose joined by a alpa-1,4-glycosidic bond and...
A: Lactose is one of the disaccharides that is mainly present in milk. It is one of the most important ...
Q: part 1 A young athlete has trained over several months to participate in a duathlon sprint. They are...
A: Hello! Thank you for the questions. As you have posted multiple questions, I would be answering the ...
Q: How to use the Kd to determine the fraction of both bound proteins? The Kd is 0.05uM. protein 1 has ...
A: In the question following quantity has been given Kd=0.05uM [P1]= 0.03 uM [P2]=0.05uM
Q: What is the principle of acrolein test? Give an examples of lipids that give positive results to acr...
A: Lipids are amphipathic molecules that take part in the synthesis of the plasma membrane. Lipids are ...
Q: One of the body's mechanisms of defense against invading bacteria is to surround them and oxidize th...
A: Plant and animal body develop various defense mechanism to fight against bacterial pathogen attack. ...
Q: Aberrant signaling through the Ras-BRaf-MAPK signal transduction pathway drives many cancers. This m...
A: Ras-BRaf-MAPK signal transduction pathway is well characterized pathway and known to transduce extra...
Q: Define the following terms in 2-3 sentences. a Glycoside b.Glycone c.Aglycone d.Bufadienolide e.Cycl...
A: Glycosides consist of two parts consisting of the glycone part which is a sugar part attached to the...
Q: Answer the problem correctly. Trust me. I'll rate you helpful. Cross my heart.
A: Given Values: The volume of the acetate buffer = 250 ml Concentration of the acetate buffer = 0.5 M ...
Q: Please answer fast What is the sequence of the coding region of sars cov 2 virus's spike protein ?
A: The largest of the four key structural proteins discovered in coronaviruses is spike (S) glycoprotei...
Q: What is an example of an unusual base pair interaction that is found in RNA but not DNA? 1. G-U ...
A: RNA is composed of nucleotides attached together via phosphodiester bonds. DNA is also composed of n...
Q: 1. What is the LDL cholesterol goal of lipid lowering? 2.Who should be treated with the statin drug...
A: Cholesterol is a fatty substance that is synthesized in the liver. There are two major types of chol...
Q: Enzymes normally enhance the rates of biochemical reactions by preferentially binding and stabilizin...
A: ATP synthase is an enzyme which form ATP by using ADP and Pi as the substrates. The enzyme is locate...
Q: P2
A: Enzyme activity is the micromole of the substrate formed per minute of the reaction. Specific activi...
Q: The diameter of a white blood cell is 16µm. Determine the diameter of the cell in cm and inches. If ...
A: Metre is the unit of length in the International System of Units (SI system) and is represented by t...
Q: Please explain the theoretical background (positive color reactions and what is being detected) invo...
A: Proteins are the polymers of amino acids. The amino acids are joined together by the peptide bonds. ...
Q: Please directly answer the theoretical background (positive color reactions and what is being detect...
A: All the given tests are qualitative tests for detection of protein. The tests are as follows: Biure...
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- (MB = methylene blue) What is the concentration of the sample of the substance with the reporter absorbance of 0.40 at 595 nm?2 a. You are trying to purify protein C from a mixture of proteins noted in the above Table. If you had only one type of column to choose from, which one would allow you to purify protein C with the least number of contaminants? Size exclusion column Ion exchange column Affinity chromatography using glucose as the bait Affinity chromatography using NAD as the bait Please explain why you chose the column above based upon the properties of the column AND the proteins in the Table.2. For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. How much of the diluent must be added to tube 2 if 200uL Red cell suspension is added? Show pertinent solutions. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions.
- 3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?1. When can gelatin be utilized in culture media preparation? 2. Why should Erlenmeyer flasks hold not more than 60% of their capacity? 3. Why is a lower concentration of NaOH or HCl used for titration while a solution with higher concentration is used for adjusting the pH of the remaining medium?2. During digestion, what happened to the sample analyte during this process? Provide the chemical reaction if possible. 3. On the analysis, what is the importance of calibrating the machine? What happened to the analyte during the analysis of AAS?
- 1. What volume of sterile water for injection (diluent) must be added to reconstitue the powder? 2. How many mL of the reconstituted cefazolin is needed to prepare the final product?6. Using a balance with a sensitivity of 4 mg, an acceptable weighing error of 5% and cherry syrup as the solvent for tartar emetic, how could you obtain the correct quantity of tartar emetic to fill the prescription?1. In the preparation of the standard curve for protein analysis, 50 mnBSA (bovine serum albumin) dissolved in H2O to a final volume of 5mL was used as stock solution. What is the weight of BSA in 0.1 mL ofstock solution? In 0.2 mL? The mentioned aliquots (#9) which were diluted with enough water to a final volume of 1 mL were assayed colorimetrically and yielded the following absorbance readings: mL stock mL H2O Absorbance0.2 0.8 0.1000.4 0.6 0.2000.6 0.4 0.3000.8 0.2 0.4001.0 0.0 0.500 i. Tabulate mg BSA vs. absorbance readingii. Draw the curve where mg BSA is on x-axis and absorbance ony-axis (use graphing paper).iii. Calculate the slope of the curveiv. If a sample solution has an absorbance of 0.332, determinemg protein of the sample.
- 3. Discuss the advantage and disadvantage of Wijs method of iodine value determination. What non-titrimetric method can be used to determine the iodine value of edible oil? Discuss its basic principles and general methods involved.The diffusivity of amino acids in polyacrylamide gel is approximately 1x10^-9 cm2./s calculate the initial flux of amino acids, give an instantaneous gradient of (20g/cm 3 )/8cm Why is polyacrylamide gel is used in electrophoresis?3. Calculate the total volume of the diluent that you are going to use if you have prepared a 3% red cell suspension using 0.5 mL of pRBC.
