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- EXPERIMENT: CRYOPRESERVATION OF CULTURED CELL LINES Table 1: The initial cell counting before cryopreservation Quadrant Death cell Live cell 1 15 0 3 7 1 5 15 0 7 15 0 9 14 0 Total 66 1 67 Table 2 : Total cells concentration before cryopreservation Total cells 67 Total viable cells 66 Percentage of viable cells (%) 98.51 Average of viable cells 13.2 Cell concentration (cells/ml) 264000 Table 3: Total cells concentration after cryopreservation Total cells 46 Total viable cells 28 Percentage of viable cells (%) 60.87 Average of viable cells 5.6 Cell concentration (cells/ml) 112000 QUESTION: Please make a conclusion in one paragraph based on the tables above.EXPERIMENT: CRYOPRESERVATION OF CULTURED CELL LINES Table 1: The initial cell counting before cryopreservation Quadrant Death cell Live cell 1 15 0 3 7 1 5 15 0 7 15 0 9 14 0 Total 66 1 67 Table 2 : Total cells concentration before cryopreservation Total cells 67 Total viable cells 66 Percentage of viable cells (%) 98.51 Average of viable cells 13.2 Cell concentration (cells/ml) 264000 Table 3: Total cells concentration after cryopreservation Total cells 46 Total viable cells 28 Percentage of viable cells (%) 60.87 Average of viable cells 5.6 Cell concentration (cells/ml) 112000 Question: From the table(result) given, please make a discussion The discussion should discuss what is cryopreservation (1), how cryopreservation is useful to make this experiment successful (2), what can be observed from total cells concentration before and after…Question:- what conditions do bacteria need to grow ? think about the condition needed for yeast and if these conditions are similar.
- Lab- testing enzyme (catalase) one patatoes why the raw patato generated a lot of air bubble after adding hydrogen peroxide (h2O2), while the cooked patato did not)Do explain. QUESTION:- If the breast cancer cells treated with CBD extract, which of the following would not be a controlled variable? a. PH buffering of the growth medium. b. Solvent used to dissolve CBD extract. c. Number of cells per plate after treatment. d. Temperature inside incubator where the cells are grown.Osmosis Lab For each beaker with the shell-less egg, identify whether the solution inside was hypotonic, hypertonic, or isotonic in comparison to the control beaker. water corn syrup corn syrup/water mixture Control no solution
- Question:- Define the bacterial categories that each type of bacteria belongs to. You only have to name the categoriesNOT the actual species of bacteria Note: there will be more than one category . A bacterial species that grows best at 37 degrees Cand when a small amount of oxygen is presentPotato osmosis lab - What do you think would happen if you placed each potato in a sucrose solution with an equal molarity to the potato?EXPERIMENT: CRYOPRESERVATION OF CULTURED CELL LINES Based on the experiment, please answer the following questions. 1. Why cryoprotectant was used to freeze the cells?2. Besides DMSO, suggest 3 other chemicals that potentially be used as cryoprotectant.3. Describe the mechanism of DMSO in shielding the cells from extreme temperature.4. In your opinion, why do we need to freeze the cells in high concentration? Justify your answer.
- Question:- Biology 1. What is the difference between scanning and electron microscopy?Activity 13 Urine Culture Inoculating Urine with a calibrated loop The number of microorganisms per milliliter recovered on urine culture can aid in the differential diagnosis of urinary tract infection (UTI). Plastic or wire inoculating loop available commercially, have been calibrated to deliver a known volume of liquid when handled correctly, thus enabling the microbiologist to estimate numbers of organism in the original specimen based on colony forming unit (CFU) of growth on cultures. PROCEDURE: 1st day 1. Gently swirl the specimen bottle to mix the urine specimen. 2. Label all plated media with name of patients, clinical specimen used and date. Label at the bottom of plates and not on the cover. 3. Obtain a disposable calibrated loop. 4. Dip the loop straight into the urine specimen so that the loop part is completely covered. Withdraw straight out. See fig. 16-18 5. Inoculate blood agar plate (BAP) as shown in fig.16-19 6. Incubate at 35 – 37˚C for 18 – 24 hours. 7. Dispose…Question:- The minimal inhibitory concentration of the antibiotic amoxicillin is 2mg/ml for Staphylococcus aureus. This means that the dose of amoxicillin needed to prevent S. aureus growth in a culture medium A. should be less than 2 mg/ml B.should be at least 2 mg/ml C. needs to be determined by an antibiogram D. is unknown as not enough information is provided