PROBLEMS8.1 An enzyme-catalysed reaction was found to be affected by two inhibitors A andB. The following results were obtained at fixed total enzyme čoncentration:Substrate conc"Initial velocity (absorbance units per minute)(mmol l-)With 1 mmol I-BUninhibitedWith 1 mmol l- A0.68450201.080.6530.468101.431.010.6490.4760.3740.31151.020.3630.7980.6573.30.2962.50.2502.00.549Comment on these results.8.2 The system investigated in problem 7.1 was investigated again under identicalconditions but in the presence of an inhibitor, giving the following data:40.06.67 10.015620.0Substrate conc" (mmol 1-1)Initial velocity (umol 1- min-1)5.0100 122222278Determine the type of inhibition. If K, for this system is 2.9 mmol 1-', calculate theinhibitor concenträtion present.

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PROBLEMS 8.1 An enzyme-catalysed reaction was found to be affected by two inhibitors A and B. The following results were obtained at fixed total enzyme concentration: Substrate conc" Initial velocity (absorbance units per minute) (mmol l-) Uninhibited With 1 mmol I-A With 1 mmol I-B 50 20 10 0.684 1.08 1.43 1.02 0.798 0.657 0.549 0.653 0.468 0.363 1.01 3.3 2.5 2.0 0.649 0.476 0.374 0.311 0.296 0.250 Comment on these results.

PROBLEMS 8.1 An enzyme-catalysed reaction was found to be affected by two inhibitors A and B. The following results were obtained at fixed total enzyme concentration: Substrate conc" Initial velocity (absorbance units per minute) (mmol l-) Uninhibited With 1 mmol I-A With 1 mmol I-B 50 20 10 0.684 1.08 1.43 1.02 0.798 0.657 0.549 0.653 0.468 0.363 1.01 3.3 2.5 2.0 0.649 0.476 0.374 0.311 0.296 0.250 Comment on these results.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter13: Enzymes—kinetics And Specificity

Section: Chapter Questions

Problem 16P

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