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- 6. Cycle of tricarboxylic acids (TCA):• importance for cellular metabolism;• intracellular localization of TCA enzymes;• sequence of TCA reactions;• TCA enzymes and coenzymes;• substrate phosphorylation reactions in TCA;• decarboxylation reactions in TCA;• enzymes and coenzymes in dihydrogen reactions in TCA;• mechanisms of regulation of TCA.22. In Exergonic reaction, all of the following are true except A. Spontaneous reaction B. Delta G is positive C. free energy of substrate is more than that of products D. catabolic reaction8. Anaplerotic and amphibolic reactions in TCA.
- Assignment on KetohexosesConstruct the structure of the following enantiomers or mirror images:1. D and L Fructose2. D and L Allulose3. D and L Sorbose4. D and L TagatoseMechanisms of catalysis: 2.5 Mechanism of chymotrypsin summary. These mechanisms involve several of the above-mentioned catalyses. In these summaries, do not just draw a diagram of the proposed mechanisms. It is more important to understand which reaction steps involve what kind of catalysis and how these help to reduce the activation energy needed for the reaction (e.g. a step in the reaction mechanism could be electrostatic catalysis to stabilise the transitions state)Instructions. Given each set of information which may include common name(s) and the reaction catalyzed, you are required to identify the main class of the specific enzyme described. _____________________ Name: alkaline phosphatase Reaction: a phosphate monoester + H2O = an alcohol + phosphate _____________________ Reaction: L-threonine = D-threonine. Other information: Inverts both chiral centers, a racemase. _____________________ Name: glycine-N-acylase Reaction: acyl-COA + glycine = CoA + N-acylglycine _____________________ Name: lysine decarboxylase Reaction: L-lysine = cadaverine + CO2 _____________________ Name: methanol dehydrogenase Reaction: methanol + NAD+ = formaldehyde + NADH + H+ _____________________ Name: citryl-CoA synthetase Reaction: ATP + citrate + CoA = ADP + phosphate + (3S)-citryl-CoA _____________________ Name: D-xylulose reductase Reaction: xylitol + NAD+ = D-xylulose + NADH + H+ _____________________ Name: cellobiose phosphorylase Reaction:…
- Instructions. Given each set of information which may include common name(s) and the reaction catalyzed, you are required to identify the main class of the specific enzyme described. _____________________ Name: alkaline phosphatase Reaction: a phosphate monoester + H2O = an alcohol + phosphate _____________________ Reaction: L-threonine = D-threonine. Other information: Inverts both chiral centers, a racemase. _____________________ Name: glycine-N-acylase Reaction: acyl-COA + glycine = CoA + N-acylglycine _____________________ Name: lysine decarboxylase Reaction: L-lysine = cadaverine + CO2 _____________________ Name: methanol dehydrogenase Reaction: methanol + NAD+ = formaldehyde + NADH + H+12. Find the ΔH for the reaction below, given the following reactions and subsequent ΔH values: b. FeO(s) + CO(g) ⟶⟶ Fe(s) + CO2(g) ΔH = __________ 3Fe2O3(s) +CO(g) ⟶⟶ 2Fe3O4 +CO2 (g) ΔH = -68.26 kJ Eq 1 Fe2O3(s) + 3CO(g) ⟶⟶ 2Fe(s) + 3 CO2(g) ΔH = -23.44 kJ Eq 2 Fe3O4(s) + CO(g) ⟶⟶ 3FeO(s) + CO2(g) ΔH = +21.79 kJ Eq 31. Consider the following parameters related to an enzyme that follows Michaelis-Menten kinetics for the reaction: k(1) k(2) S ----> ES ----> P k(-1)
- Analysis of the Temperature-Dependent Term of the rate of reaction Provide at least 3 chemical equations with a given activation energy and frequency factor. (endothermic reactions, exothermic reactions, reactions with high and low Eact , reactions with catalyst) provide equation problems only. no need to solve it.12. Explain why those biological reactions that have their equilibria shifted towards theproducts have negative values for ΔGo of reactions. Explain how equilibria relates toGibbs free energy.Result nad Discussion Lead Acetate Reaction: Samples: lysine, cysteine, methionine Reagents: 10% Sodium Hydroxide (NaOH) and Lead Acetate Pb(CH3COO)2 -To 1 ml of the amino acid solution taken in a test tube, add few drops of sodium hydroxide (40%) and boil the contents for 5-10 mins over a bunsen burner. Cool the contents and add few drops of 10% Lead acetate solution and observe.
