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- Comparing Glycolysis Entry Points for Sucrose Sucrose can enter glycolysis by either of two routes: Sucrose phosphorylase: Sucrose + Pi fructose + glucose-1-phosphate Invertase: Sucrose + H20 fructose + glucose Would either of these reactions offer Jin advantage over the other In the preparation of hexoses fur entry into glycolysis?The Reactions and Meehanisms of the Leloir Pathway Write the reactions that permit galactose to be utilized in glycolysis. Write a suitable mechanism, tor one of these reactions.Complete oxidation of a 16-carbon fatty acid can yield 129 molecules of ATP Study Figure 19.2 and determine how many ATP molecules would be generated if a 16-carbon fatly acid were metabolized solely by the TCA cycle, in the form of S acetyl-CoA molecules.
- Understanding Enzyme Mechanisms Related to Pyruvate Carboxylase Based on the mechanism for pyruvate carboxylase (Figure 22.3), write reasonable mechanisms for the reactions that follow:The Effect of lodoacetic Acid on the Glyceraldehyde-3-P Dehydrogenase Reaction (Integrates with Chapters 4 and 14.) How might iodoacetic acid affect the glyceraldehydes-3-phosphate dehydrogenase reaction in glycolysis? Justify your answer.threose-4 phosphate (a 4 carbon aldose) is fed into glycolysis after a decarboxylation reaction that uses an NAD+ to generate a 3 carbon aldose. How many ATP and NADH are generated by breaking down this molecule to pyruvate?
- Fatty acid degradation stimulates the citric acid cyclethrough the activation of pyruvate carboxylase by acetylCoA. Why would the activation of pyruvate carboxylaseincrease energy generation from fatty acids?Thioester play an important role in glycolysis and the TCA cycle. Which reactions in glycolysis and tca cycle involve thioesters.Considering the fatty acids: (a) Arachidic acid (C20H40O2); molar mass = 312.5 g/mol) (b) Palmitoleic acid (C16H30O2); molar mass = 256.4 g/mol). How many cycles of β -oxidation are needed for complete oxidation? How many molecules of acetyl CoA are formed from its complete catabolism? How can you calculate the number of molecules (moles) of ATP formed (net) by the complete catabolism of each fatty acid? and the number of moles of ATP formed per gram of each fatty acid metabolized??
- Glycolysis After digestion of carbohydrates, glucose enters the cell membrane and into the cytoplasm, glycolysis takes place where glucose-6-phosphate (a 6C-molecule) is split into two 3C molecules of gly-3-P, producing 2 pyruvates. The two pyruvates are then converted to two acetyl-CoA and enter the mitochondria where the citric acid occurs. 1. What reactions are involved in the so called, energy-investing reactions? What chemical compound is needed to initiate the reaction? 2. Cite the steps in the glycolysis pathway where these energy-investing reactions occur. 3. The conversion from glu-6-P to fru-6-P involves isomerization, why? 4. What stages in the glycolysis pathway are considered energy harvesting reactions? What are the products of these reactions that will prove its energy yield? 5. The conversion from gly3-P to 1,3-diP uses NAD+ and a dehydrogenase enzyme. What is the role of NAD+? What reactions therefore are involved in these conversions? 6. The conversion…the first two reactions in glycolysis associated with unfavorable ∆G° values, i.e., ∆G° > 0, both produce a product with high phosphoryl group transfer potential. (ATP formation is not considered here.) Compare the two reactions and describe what the driving force is for both reactions that allows glycolysis to proceed to formation of pyruvate.Gluconeogenesis o How are the waterfall reaction of glycolysis bypassed? o Know the structure of oxaloacetate