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Q14) Interested in studying ethanol fermentation, you prepare two solutions of yeast extract that contains the cytosolic contents of Saccharomyces cerevisiae. You add glucose to both extracts at time zero, but to one (in red) you also add inorganic phosphate (added in two batches). Using a gas sensor, you monitor the CO2 generated by the two solutions over time and obtain the following data:

Q14) Interested in studying ethanol fermentation, you prepare two solutions of yeast extract that contains the cytosolic contents of Saccharomyces cerevisiae. You add glucose to both extracts at time zero, but to one (in red) you also add inorganic phosphate (added in two batches). Using a gas sensor, you monitor the CO2 generated by the two solutions over time and obtain the following data:

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Q14) Interested in studying ethanol fermentation, you prepare two solutions of yeast extract that contains the cytosolic contents of Saccharomyces cerevisiae. You add glucose to both extracts at time zero, but to one (in red) you also add inorganic phosphate (added in two batches). Using a gas sensor, you monitor the CO2 generated by the two solutions over time and obtain the following data: + additional Pi +Pi no Pi added Time (min) a) Why is more CO2 generated upon the addition of phosphate? b) During fermentation, what would the ratio of CO2 generated to phosphate added expected to be? c) During fermentation, what would the ratio of CO2 generated to glucose consumed expected to be? d) You become worried that the flasks would burst open to the build up in gas pressure. What other molecules could you add to the extract to slow down CO2 generation even in the presence of glucose and Pi? CO, generated (mmoles)
Transcribed Image Text:Q14) Interested in studying ethanol fermentation, you prepare two solutions of yeast extract that contains the cytosolic contents of Saccharomyces cerevisiae. You add glucose to both extracts at time zero, but to one (in red) you also add inorganic phosphate (added in two batches). Using a gas sensor, you monitor the CO2 generated by the two solutions over time and obtain the following data: + additional Pi +Pi no Pi added Time (min) a) Why is more CO2 generated upon the addition of phosphate? b) During fermentation, what would the ratio of CO2 generated to phosphate added expected to be? c) During fermentation, what would the ratio of CO2 generated to glucose consumed expected to be? d) You become worried that the flasks would burst open to the build up in gas pressure. What other molecules could you add to the extract to slow down CO2 generation even in the presence of glucose and Pi? CO, generated (mmoles)
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