Q27 A 10-1 is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 265 colonies of bacteria grow on the plate. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer as a whole number. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.
Q27 A 10-1 is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 265 colonies of bacteria grow on the plate. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer as a whole number. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter32: Radiochemical Methods
Section: Chapter Questions
Problem 32.18QAP: The streptomycin in 500 g of a broth was determined by addition of 1.25 mg of the pure antibiotic...
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Q27
A 10-1 is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 265 colonies of bacteria grow on the plate.
Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer as a whole number.
- Since only 0.1 mL is put on the plate, this counts as an extra dilution!!!
- Any time less than 1 mL is transfered, a dilution is being performed.
- Any time more than 1 mL is transfered, a concentration is being performed.
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