QUESTION 1 As your first experiment as a graduate student, your advisor asks you to reconstitute purified bacteriorhodopsin, a light- driven H+ pump from the plasma membrane of photosynthetic bacteria, and purified ATP synthase from ox-heart mitochondria together in the same membrane vesicles, as shown on the right. You work out a method so that all of the bacteriorhodopsin pumps H+ in one direction, into the vesicles. You then add ADP and Pi to the external medium and shine light onto the suspension of vesicles. What do you observe? purified ATP synthase purified bacteriorhodopsin detergent ADD PHOSPHOLIPIDS AND REMOVE DETERGENT LIGHT sealed vesicle (liposome) ete Garland Science 2010) A No ATP synthesis since no electron transport occurs. Figure 014-18 Esential Cell Biolegy le e B. ATP synthase makes ATP. CATP synthase hydrolyzes ATP to pump H+ into the vesicles. D.No ATP synthesis since no H+ gradient is created.

Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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QUESTION 1
As your first experiment as a graduate student, your advisor asks you to reconstitute purified bacteriorhodopsin, a light-
driven H+ pump from the plasma membrane of photosynthetic bacteria, and purified ATP synthase from ox-heart
mitochondria together in the same membrane vesicles, as shown on the right. You work out a method so that all of the
bacteriorhodopsin pumps H+ in one direction, into the vesicles. You then add ADP and Pi to the external medium and shine
light onto the suspension of vesicles. What do you observe?
purified ATP
synthase
purified
bacteriorhodopsin
detergent
ADD PHOSPHOLIPIDS
AND REMOVE
DETERGENT
LIGHT
sealed vesicle
(liposome)
Figure Q14-18 Essential Cell Biology ale te Garland Science 2010)
A NO ATP synthesis since no electron transport occurs.
B. ATP synthase makes ATP.
CATP synthase hydrolyzes ATP to pump H+ into the vesicles.
D.No ATP synthesis since no H+ gradient is created.
Click Save and Submit to save and submit. Click Save All Answers to save all answers.
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...
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MacBook Pro
Q Search or enter websito namo
Transcribed Image Text:QUESTION 1 As your first experiment as a graduate student, your advisor asks you to reconstitute purified bacteriorhodopsin, a light- driven H+ pump from the plasma membrane of photosynthetic bacteria, and purified ATP synthase from ox-heart mitochondria together in the same membrane vesicles, as shown on the right. You work out a method so that all of the bacteriorhodopsin pumps H+ in one direction, into the vesicles. You then add ADP and Pi to the external medium and shine light onto the suspension of vesicles. What do you observe? purified ATP synthase purified bacteriorhodopsin detergent ADD PHOSPHOLIPIDS AND REMOVE DETERGENT LIGHT sealed vesicle (liposome) Figure Q14-18 Essential Cell Biology ale te Garland Science 2010) A NO ATP synthesis since no electron transport occurs. B. ATP synthase makes ATP. CATP synthase hydrolyzes ATP to pump H+ into the vesicles. D.No ATP synthesis since no H+ gradient is created. Click Save and Submit to save and submit. Click Save All Answers to save all answers. Save All Answe NOV ... 21 MacBook Pro Q Search or enter websito namo
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