QUESTION 1 Which of the following concepts in column A best fits the concept in column B. p-Nitrophenyl a-D-Glucoside Action of intracellular a-GLUCOSIDASE + NaHCO3 + Boiling cells + Tris-pH 5.0 A. Substance that act as molecular mimic of a maltose molecule B. Develops TT-Nitrophenyl into a yellow colour reagent for measurement at 400nm c. Breaks the a-linkages of p-Nitrophenyl a-D-Glucoside, leaving p-Nitrophenyl and a glucose molecule D. Allows transport of p-Nitrophenyl a-D-Glucoside across the membrane E. Stop the reaction and release internal p-Nitrophenyl

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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QUESTION 1
Which of the following concepts in column A best fits the concept in column B.
p-Nitrophenyl a-D-Glucoside
Action of intracellular a-GLUCOSIDASE
+ NaHCO3
Boiling cells
Tris-pH 5.0
A. Substance that act as molecular mimic of a maltose
molecule
B. Develops TT-Nitrophenyl into a yellow colour reagent for
measurement at 400nm
c. Breaks the a-linkages of p-Nitrophenyl a-D-Glucoside,
leaving p-Nitrophenyl and a glucose molecule
D. Allows transport of p-Nitrophenyl a-D-Glucoside across
the membrane
E. Stop the reaction and release internal p-Nitrophenyl
Transcribed Image Text:QUESTION 1 Which of the following concepts in column A best fits the concept in column B. p-Nitrophenyl a-D-Glucoside Action of intracellular a-GLUCOSIDASE + NaHCO3 Boiling cells Tris-pH 5.0 A. Substance that act as molecular mimic of a maltose molecule B. Develops TT-Nitrophenyl into a yellow colour reagent for measurement at 400nm c. Breaks the a-linkages of p-Nitrophenyl a-D-Glucoside, leaving p-Nitrophenyl and a glucose molecule D. Allows transport of p-Nitrophenyl a-D-Glucoside across the membrane E. Stop the reaction and release internal p-Nitrophenyl
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