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Question 3. The nutrient broth is a basic media used for growing a broad variety of microorganisms in the laboratory. The nutrient broth consists mainly of 1.5 gL' beef extract, 3gL" yeast extract and 5 gL' sodium chloride dissolved in distilled water. The nutrient agar is prepared by adding the agar at the desired amount into nutrient broth. Answer the following questions. a) How would you prepare a 700 ml nutrient broth? b) How would you prepare 2.5% (w/v) nutrient agar for 1L? c) How would you prepare a 100 ml nutrient broth supplemented with 50 ug/ml ampicillin antibiotic? The stock concentration for ampicillin solution is 100 mg/ml.

Question 3. The nutrient broth is a basic media used for growing a broad variety of microorganisms in the laboratory. The nutrient broth consists mainly of 1.5 gL' beef extract, 3gL" yeast extract and 5 gL' sodium chloride dissolved in distilled water. The nutrient agar is prepared by adding the agar at the desired amount into nutrient broth. Answer the following questions. a) How would you prepare a 700 ml nutrient broth? b) How would you prepare 2.5% (w/v) nutrient agar for 1L? c) How would you prepare a 100 ml nutrient broth supplemented with 50 ug/ml ampicillin antibiotic? The stock concentration for ampicillin solution is 100 mg/ml.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Question 3. The nutrient broth is a basic media used for growing a broad variety of microorganisms in the laboratory. The nutrient broth consists mainly of 1.5 gL' beef extract, 3gL" yeast extract and 5 gL sodium chloride dissolved in distilled water. The nutrient agar is prepared by adding the agar at the desired amount into nutrient broth. Answer the following questions. a) How would you prepare a 700 ml nutrient broth? b) How would you prepare 2.5% (w/v) nutrient agar for 1L? c) How would you prepare a 100 ml nutrient broth supplemented with 50 ug/ml ampicillin antibiotic? The stock concentration for ampicillin solution is 100 mg/ml.
Transcribed Image Text:Question 3. The nutrient broth is a basic media used for growing a broad variety of microorganisms in the laboratory. The nutrient broth consists mainly of 1.5 gL' beef extract, 3gL" yeast extract and 5 gL sodium chloride dissolved in distilled water. The nutrient agar is prepared by adding the agar at the desired amount into nutrient broth. Answer the following questions. a) How would you prepare a 700 ml nutrient broth? b) How would you prepare 2.5% (w/v) nutrient agar for 1L? c) How would you prepare a 100 ml nutrient broth supplemented with 50 ug/ml ampicillin antibiotic? The stock concentration for ampicillin solution is 100 mg/ml.
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