Question 8 You have performed a peptide conjugation reaction in which the peptide shown below forms four types of products (1-mer, 2-mer, 3-mer and 4-mer). To separate these products you performed a column chromatography in aqueous solution at pH=6. You got the elution profile shown on the plot. a. WHAT KIND OF CHROMATOGRAPHY did you perform and WHY? b. Determine the MONOMER:DIMER MOLAR RATIO in your reaction. EXPLAIN how you arrived at your number.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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Question 8
You have performed a peptide conjugation reaction in which the peptide shown below forms four types of
products (1-mer, 2-mer, 3-mer and 4-mer). To separate these products you performed a column
chromatography in aqueous solution at pH-6. You got the elution profile shown on the plot..
a. WHAT KIND OF CHROMATOGRAPHY did you perform and WHY?
b. Determine the MONOMER:DIMER MOLAR RATIO in your reaction. EXPLAIN how you arrived at your
number.
A280
Peptide
GSGAGGPGLGGISNAAW
4
3.5
3
2.5
0.5
0
B
Mii
time
Paragraph
BI U A
E E
V
O
a
+ v
....
Transcribed Image Text:Question 8 You have performed a peptide conjugation reaction in which the peptide shown below forms four types of products (1-mer, 2-mer, 3-mer and 4-mer). To separate these products you performed a column chromatography in aqueous solution at pH-6. You got the elution profile shown on the plot.. a. WHAT KIND OF CHROMATOGRAPHY did you perform and WHY? b. Determine the MONOMER:DIMER MOLAR RATIO in your reaction. EXPLAIN how you arrived at your number. A280 Peptide GSGAGGPGLGGISNAAW 4 3.5 3 2.5 0.5 0 B Mii time Paragraph BI U A E E V O a + v ....
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