Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Unknown final buret reading (mL) 26.33 30.29 33.21 35.10 36.22 46.69 initial buret reading (mL) 21.74 26.33 30,29 33.21 35.10 45.70 volume dispensed (mL) final buret reading (mL) 7.60 9.41 12.45 16.39 25.10 initial buret reading (mL) 6.55 7.60 9.41 12.45 16.39 volume dispensed (mL) [dye] (M) 0.000048 Abs (blue light) 0.235 0.193 0.151 0.092 0.0638 0.148 CALCULATIONS 1. Create a Beer-Lambert plot using a spreadsheet program such as Excel. Plot concentration of dye (y- axis) versus absorbance at 2mar (x-axis). Add a linear trendline based on your data. Also show the equation of the line and R2-value on the graph. water dye stock solution

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter1: Introduction
Section: Chapter Questions
Problem 1.11QAP
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Absorption Spectroscopy
** SUBMIT YOUR STANDARD CURVE WITH THIS REPORT SHEET
Sample 1
Sample 2
Sample 3
Sample 4
Sample 5
Unknown
final buret reading
(mL)
26.33
30.29
33.21
35.10
36.22
46.69
initial buret reading
(mL)
21.74
26.33
30.29
33.21
35.10
45.70
volume dispensed
(mL)
final buret reading
(mL)
7.60
9.41
12.45
16.39
25.10
initial buret reading
(mL)
6.55
7.60
9.41
12.45
16.39
volume dispensed
(mL)
[dye] (M)
0.000048
Abs (blue light)
0.235
0.193
0.151
0.092
0.0638
0.148
CALCULATIONS
Create a Beer-Lambert plot using a spreadsheet program such as Excel. Plot concentration of dye (y-
axis) versus absorbance at 2max (x-axis). Add a linear trendline based on your data. Also show the
equation of the line and R2-value on the graph.
1.
water
dye stock solution
Transcribed Image Text:Absorption Spectroscopy ** SUBMIT YOUR STANDARD CURVE WITH THIS REPORT SHEET Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Unknown final buret reading (mL) 26.33 30.29 33.21 35.10 36.22 46.69 initial buret reading (mL) 21.74 26.33 30.29 33.21 35.10 45.70 volume dispensed (mL) final buret reading (mL) 7.60 9.41 12.45 16.39 25.10 initial buret reading (mL) 6.55 7.60 9.41 12.45 16.39 volume dispensed (mL) [dye] (M) 0.000048 Abs (blue light) 0.235 0.193 0.151 0.092 0.0638 0.148 CALCULATIONS Create a Beer-Lambert plot using a spreadsheet program such as Excel. Plot concentration of dye (y- axis) versus absorbance at 2max (x-axis). Add a linear trendline based on your data. Also show the equation of the line and R2-value on the graph. 1. water dye stock solution
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