Select all that apply to a negative stain: O uses only 1 dye in the procedure uses multiple dyes in the procedure O involves fixing O does not involve fixing O involves a washing step does not involve a washing step uses a basic or positively charged dye which stains the bacterial cells Ouses an acidic or negatively charged dye which stains the background can show cell morphology, size, and arrangement

Select all that apply to a negative stain: O uses only 1 dye in the procedure uses multiple dyes in the procedure O involves fixing O does not involve fixing O involves a washing step does not involve a washing step uses a basic or positively charged dye which stains the bacterial cells Ouses an acidic or negatively charged dye which stains the background can show cell morphology, size, and arrangement

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Select all that apply to a negative stain: 1. involves a washing step 2. cells may be distorted or shrunken 3. uses an acidic or negatively charged dye which stains the background 4. uses multiple dyes in the procedure 5. uses only 1 dye in the procedure 6. involves fixing 7. does not involve fixing 8. cells will not be distorted or shrunken 9. does not involve a washing step 10. can show cell morphology, size, and arrangement 11. uses a basic or positively charged dye which stains the bacterial cells
Describe in detail all the steps needed to determine whether an Unknown bacteria has a Gram-positive or Gram-negative cell wall using the Gram stain. (You can describe the method presented in the Exercise 3-6 Gram stain videos or in the Lab Manual.) 1. Start by describing the method of transferring the bacteria onto the microscope slide, then describe heat-fixing the emulsion of the Unknown bacteria. (Do not describe all the steps needed to perform aseptic technique. Just say “Bacteria was added aseptically.”) 2. Describe the Gram stain in detail. (This description should include all the stains, how long the stain should set on slide, and Genus and species names of bacteria you are using as CONTROLS.)
The presence of a capsule around bacterial cells usually indi-cates their increased disease-causing potential and resistance todisinfection. Capsules are generally viewed by:(a) Spore staining(b) Scanning electron microscopy(c) Gram staining(d) Ziehl-Neelsen staining(e) Negative staining
Janet is doing a Gram stain on a mixed culture that is known to contain both Gram positive and Gram negative bacteria. After finishing, she realizes she mixed up the safranin and alcohol steps. What is likely to be observed when she views the slide under the microscope? All cells will appear purple, regardless of whether they are actually Gram negative or Gram positive. All cells will appear pink, regardless of whether they are actually Gram negative or Gram positive. Gram negative cells will be clear, Gram positive cells will be purple. Gram positive cells will be clear, Gram negative cells will be pink. Cells will appear clear, regardless of whether they are actually Gram negative or Gram positive.
Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of simple staining and gram staining. 1. Simple Staining
Which of the following can be used to distinguish one colony from another on an agar plate? Select all that apply. a. Shape b. Color c. Gram stain d. Cell arrangement e. Size f. Elevation g. Margin h. Texture
observe the transparent bacteria under bright field microscope, we use a. Simple staining b. Gram staining c. Negative staining d Direct staining eAll the above
Cellular stains are commonly used in bacteriology. Which of the following is NOT a reason to stain a bacterial preparation. Cellular stains are commonly used in bacteriology. Which of the following is NOT a reason to stain a bacterial preparation. Determine metabolic properties of a cell. To determine the shape of the bacteria in the culture. To determine what type of cell wall the bacteria has (e.g. gram positive or Gram negative) Identify specific features of a cell (spores, flagella etc)
Which of the following describes the usefulness of a Gram stain. Select all that apply. a. A Gram stain can be used to check for bacteria at the site of a suspected infection or in certain body fluids, such as blood or urine. b. A Gram stain can be used to determine whether penicillin will be effective at treating the patient or whether a different antibiotic should be used. c. A Gram stain can determine whether the bacteria have a thick or thin layer of peptidoglycan in their cell wall.
Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of simple staining and gram staining. 2. Gram Staining
which statemts pertin to Gram stain, acid-fast stain, or spore stain. please group statements with the correct term they pertain to Here are the descriptions: -does not require heat to be used in staining process -distinguishes between active metabolic cells and and dormant structures -a chemical is used as a mordant -differentiates cell with high lipid content in cell wall -uses carbol fuchsin, acid alcohol and methylene blue uses malachite green -differentitates cells based on thickness of peptidoglycan layer -result is purple and red/pink cells -important diagnostic tool in mycobacterium infections
Gram straining procedure. Indicate the following information A. Shape........ B. Stained color under microscope........ C. PG Wall thick, thin or both.....