Select the correct order of steps in a polymerase chain reaction. create reaction mixture, denaturation, extension, primer annealing denaturation, create reaction mixture, primer annealing, extension create reaction mixture, denaturation, primer annealing, extension denaturation, create reaction mixture, extension, primer annealing

Select the correct order of steps in a polymerase chain reaction. create reaction mixture, denaturation, extension, primer annealing denaturation, create reaction mixture, primer annealing, extension create reaction mixture, denaturation, primer annealing, extension denaturation, create reaction mixture, extension, primer annealing

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter14: Biotechnology And Society

Section: Chapter Questions

Problem 9QP

See similar textbooks

Similar questions

What goes into the reaction tube for Sanger sequencing? Template DNA, primers, DNA polymerase, and fluorescent dNTPs. Template DNA, primers, DNA polymerase, dNTPs, and fluorescent ddNTPs. Primers, dNTPs, and fluorescent ddNTPs.
Which of the following is true regarding next generation sequencing? 1. Next generation sequencing is also known as sequencing by synthesis 2. Next generation sequences uses DDNTPS to identify the base at the end of the nucleotide chain 3. Next generation sequencing will not work if chain terminating nuclear tides are included in the reaction 4. Next generation sequences uses fluorescently labeled ddNTPs instead of radioactivity labeled ddNTPs 5. Next generation sequencing allows hundreds of millions of DNA fragments to be sequenced at the same time
List the steps in the polymerase chain reaction; discuss one disadvantage to this technique
The following image is of an agarose gel. If DNA samples were loaded to this gel and the electrophoresis experiment was started, explain what would happen and why.
When half of the DNA in the sample is double-stranded, that point is termed the half- reaction time (C0t1/2). Plot a graph below that describes how genome sizes of MS2, T4 and E.coli affects the half-reaction time.
From the following DNA template, which sequence is synthesized by RNA Polymerase? 5’- T – C – C – T – A – A – G – A –3’ Group of answer choices 5’- A – G – G – A – T – T – C – T –3’ 5’- U – C – C – U – A – A – G – A –3’ 3’- A – G – G – A – U – U – C – U –5’ 3’- T – C – C – T – A – A – G – A –5’ 3’- T – G – G – A – T – T – C – T –5’
Please write down the DNA sequence inferred from the below DNA gel. Shown are the products of a dideoxy sequencing reaction.
A = double cutter (1000 bp, 800 bp) - 800 is being cut by the single cutter because 600 + 200 = 800, 1000 is intact B = single cutter (2000 bp) A/B (1000 bp, 600 bp, 200 bp) Using the plasmid map identify which enzyme is the single and double cutter
Which of the following statements is true of a Sanger sequencing reaction but not colony PCR? Select all that apply. Must contain only a single primer ddNTPs are added Uses a heat stable DNA polymerase Requires a pair of primers that bind to opposite strands Requires temperature cycling
Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.
What do you think are the crucial steps in Polymerase Chain Reaction technique? Enumerate the advantage and disadvantage of Polymerase Chain Reaction technique? What are the things to consider in making a good primer? Does temperature affects the primer design? Discuss briefly.
Describe the function of the following reagents used in the DNA extraction procedure?a) Proteinase K b) 5M Nacl c) Isopropanol d) 1X TE Buffer