Separate a mixture of Vitamin D and Vitamin B2 (riboflavin) by paper chromatography. The stationary phase is methanol (CH3OH) and the mobile phase is pentane (CSH12). Place the vitamins in order of elution. Explain why using IMF. (Circle polar sites on each molecule and label all 4 molecules as polar or non-polar.) Vitamin B H3C H,C CH CH, H,C он CH2 Vitamin D OH CH,OH
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- 4) If the classification of the chromatography run is a reverse-phase chromatography, discuss the relative polarities of caffeine, aspartame and benzoate.5) Given the data of the diet sodas, which one would you recommend to be the healthiest? (You may need to research the functions of caffeine, aspartame and benzoate. Please add references if necessary.)Enumerate at least two important things to do to maximize yield when using vacuum filtration in the synthesis of aspirin.Classify the descriptions based on whether they apply to ion‑exchange, size‑exclusion, or both types of chromatography columns. - Proteins move through the column at rates determined by their net charge. - The gel bed will contain polymer beads with charged functional groups. - Proteins move through the column at rates determined by their size. - The gel bed will contain porous polymer beads. - The properties of the proteins affect the rate at which they migrate. - Proteins are separated based on differences in their amino acid sequences.
- Consider the following statements as they apply to gas chromatography. Decide if each is true or false. 1. ___ Temperature programming is necessary only for samples containing compounds with a narrow range of boiling points. 2. ___ GC carrier gases are generally inert gases like helium and nitrogen. 3. ___ The injection port temperature should be set at the average boiling point of all analytes. 4. ___ An FID is a mass-sensitive detector, not a concentration-sensitive device. 5. ___ An air peak is commonly used to determine the dead time when using an FID detector.in a chromatographic analysis of water from a drinking fountain gives area of 1.56*10^4, 5.12*10^4, 1.49*10^4 and 1.76*10^4 for CH3CL3, CHCL2BR, CHCLBR2 and CHBR3 respectively. determine the percentage concentration of each of the triichoromethane in the sampleWhich of the following statement is false? a. Component with the strong attraction to the support move more slowly than component with weak attraction. b. Chromatography technique used to separate only colored compounds. c. On TLC, single spot does not mean or guarantee a single substance (compound). d. Iodine vapor and UV- lamp are methods used for visualization uncolored compounds.
- A spot of crude plant extract was placed on a glass plate coated with C18 silica gel. The chromatogram was developed using an appropriate solvent. The result is shown below. Which of the following spot is the most soluble in water? Choices: Spot C Spot M Spot E Spot HBased on this. What was the percentage error in the percentage of water in the sample, compared to pure MgSO4•7H2O?Which statement is true? Statement 1: In chromatography, the compound mixture moves along with the mobile phase through the stationary phase and the components get separated depending on the degree of interaction and adhesion Statement 2: Compound A is more soluble in the mobile phase compared to compound B. Thus, compound A would most probably have a higher Rf value.
- 1. Are there any difference between using a 2% NaCl and using a water in the paper chromatography. If there are, what is the differences between the two? If they are the just the same, state the reasons.What is the function of a desiccant in a set-up of volatilization gravimetry? To remove nitrogen from the sample To remove sulfuric acid from the sample To remove water vapor from the sample To remove carbon dioxide from the sampleImagine you are attempting to separate these three dipeptides from each other using anion exchange chromatography, and you use a protein assay to identify at what salt concentration each of them is eluted from the column. A graph showing protein concentration as a function of salt concentration is shown below the dipeptides. Which of the three dipeptides is represented by peak #3? (NOTE: the dipeptides are shown in their non-ionized forms. You must assume that they are in aqueous solution at physiological pH, ~7.2) a) Dipeptide A b) Dipeptide B c) Dipeptide C