SHOW COMPLETE SOLUTIONSQ3. Which isolate has A260/280 ratio of above 2.0? The purity and concentration of DNA isolate can be evaluated with the use of UVspectrophotometeric measurements. Absorbance reading at 320 provides a generalmeasurement of the turbidity of the sample. The organic compounds (containing aromaticrings) used as reagents to extract DNA absorb light strongly at 230. The ideal 260/230 ratiois 2.0 -2.2. DNA absorbs light most strongly at 260nm so the absorbance value at thiswavelength can be used to estimate the DNA concentration using the equation derived fromBeer's Law:Concentration (µg/mL) = (A260 reading - A320 reading) x 50The absorbance at 280nm is used as an indicator of protein contamination since thearomatic amino acid residues absorb strongly at this wavelength.Analyze the data given below and determine the following:Isolate AIsolate BIsolate CIsolate DA3200.0510.0910.0650.073A2301.271.321.951.44A2604.543.923.884.21A2802.012.112.042.32

Given that, a table of absorbance at different wavelength of different isolates is shown below We…

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SHOW COMPLETE SOLUTIONS Q3. Which isolate has A260/280 ratio of above 2.0?

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter17: Applications Of Infrared Spectrometry

Section: Chapter Questions

Problem 17.9QAP

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Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.

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SHOW COMPLETE SOLUTIONS

Q3. Which isolate has A260/280 ratio of above 2.0?

The purity and concentration of DNA isolate can be evaluated with the use of UV
spectrophotometeric measurements. Absorbance reading at 320 provides a general
measurement of the turbidity of the sample. The organic compounds (containing aromatic
rings) used as reagents to extract DNA absorb light strongly at 230. The ideal 260/230 ratio
is 2.0 -2.2. DNA absorbs light most strongly at 260nm so the absorbance value at this
wavelength can be used to estimate the DNA concentration using the equation derived from
Beer's Law:
Concentration (µg/mL) = (A260 reading - A320 reading) x 50
The absorbance at 280nm is used as an indicator of protein contamination since the
aromatic amino acid residues absorb strongly at this wavelength.
Analyze the data given below and determine the following:
Isolate A
Isolate B
Isolate C
Isolate D
A320
0.051
0.091
0.065
0.073
A230
1.27
1.32
1.95
1.44
A260
4.54
3.92
3.88
4.21
A280
2.01
2.11
2.04
2.32

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