Since KOH can also determine whether a culture is Gram positive or negative, why go through all the trouble of doing a Gram stain instead? part 2 What causes the KOH test to work? -part 3 How do you know a KOH test has identified a gram negative? Gram positive?
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Q5) Since KOH can also determine whether a culture is Gram positive or negative, why go through all the trouble of doing a
part 2 What causes the KOH test to work?
-part 3 How do you know a KOH test has identified a gram negative? Gram positive?
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- a. After conducting the Gram staining procedures and you observed that all cells are stained with too much purple though the bacteria you fixed is Gram negative, what could be the mistake you have done? b. After conducting the Gram staining procedures and you observed that all cells are stained with too much pink though the bacteria you fixed is Gram positive, what could be the mistake you have done?Answer this question xi) What is the most important step in the Gram staining procedure? Why?Q5) Since KOH can also determine whether a culture is Gram positive or negative, why go through all the trouble of doing a Gram stain instead? - What causes the KOH test to work? - How do you know a KOH test has identified a gram negative? Gram positive?
- list 4 advantages and 4 limitations of the Gram staining process1.)What is the purpose of a counterstain? 2. What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant? 3. True or False? The oil objective should make contact with the oil on the slide. 4. Why is it necessary to let bacterial smears completely air dry before heat fixing? 5.Why should controls be included wherever possible for any staining technique? 6. Why is it necessary to heat the slides while staining for endospores?Write down 4 ways to get false positives and false negatives while Gram staining.
- Hi , First Test Gram stain is Gram negative, and in order to find that what other possible test can be performed ? For example - Oxidation Test for test 2. . And for 3,4,5 and 6 ? What other possible test can be performed? Thank you !13. A student makes a smear by applying a LARGE amount of bacteria onto the slide. A simple stain is performed, but when the student looks under the microscope, individual organisms can’t be distinguished. What did the student do wrong when making the smear?Which step in the Gram stain procedure is most prone to error? If done incorrectly, how might that step affect the end result?
- (a) Which would be easier to prepare bacterial smear from, liquid or solid culture? Why? (b) Aside from bacteria, can gram staining be used with microbes such as fungi? Explain your answer.Why should a young culture be used in gram staining rather than an old culture?b. Why is it necessary to decolorize only for a few seconds1.What advantage(s) does the pour plate method have over the streak-plate method? 2.Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the inoculation? 3.Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled to 50° C? 4.Explain why plates should be inverted during incubation. 5.Explain why plates should be inverted during incubation.