Why do you think rapid methods so helpful to identify pathogens microbes in clinical settings. Why are traditional test such as metabolic test not always used to ID clinically important microbes
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Why do you think rapid methods so helpful to identify pathogens microbes in clinical settings.
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- A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. Which type of bacteria would be used to test the autoclave?A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. What must be done with the bacterial sample after it is processed in the sterilizer?A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. Which characteristic of the species used determines whether sterility was achieved when autoclaved?
- You have learned many different techniques so far this semester that allow us to identify pathogenic microbes in the clinical setting. Why do you think rapid methods are so helpful? Why are traditional tests such as metabolic tests not always used to ID clinically important microbes?Why are traditional tests such as metabolic test not always used to ID clinically important microbes?In the Kirby-Bauer test, which of the following must be consistent? a. concentration of bacteria placed on the plate b. type of medium in the plate c. concentration of antimicrobial drug in the disk d. all of these
- Which isolation technique is best used in isolating microorganisms (in countable good plates) from a gram of soil sample with expectedly high microbial count? A. Streak Plate technique B. Serial Dilution-Pour Plate technique C. Spread Plate technique D. Hanging Drop technique 2. Which materials are needed in isolating bacteria using Streak Plate technique? Check all that apply. ( )nutrient agar plate ( ) inoculating loop ( ) microbial inoculum ( ) flame source 3. Which term refers to that solution of sterile distilled water/normal saline/peptone water used to "thin out" dense microbial populations in Serial Dilution-Pour Plate technique used in the isolation of microorganisms? A. inoculum B. culture medium C. diluent D. microbial cultureAfter streaking microbial culture on agar plates and observing colonial growth, TMTC usually happens. What are the causes of TMTC plates (plates with more than 300 colonies that cannot be counted)? What are the ways to prevent this from happening?In spread plate method, inoculating loop is used to spread bacteria from the inoculation site. For quadrant streaking, the loop must be flame sterilized between quadrant streaks. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.
- There are so many microbes in a single mL of culture, it is very difficult to perform one dilution to produce countable cells. Microbiologists need to perform a dilution series, where multiple dilutions are performed in sequence to arrive at the correct dilution. Dilutions are cumulative. Multiple the series of dilutions together to find the final dilution value. If 3 serial dilutions are performed, each with a value of 0.01, what is the cumulative dilution? Express your answer as an exponent, e.g. 0.1 would be 1e-1 and 0.01 would be 1e-2Name three reasons why the ELISA test is useful in detecting microbesWhich of the following is not meant to reduce contamination of yoru sample? avoiding hand contact with the speciman none of these heating the inoculation loop until red heat fixation of the speciman flaming the lip of a test tube culture
