Smear Preparation and Staining LabSmear Preparation1. What are two goals of a good smear?2. Why does a bacterial smear need to completely air dry prior to heat fixing?3. What is the purpose of heat fix step?Simple Stain1. What is the difference between simple stain and complex stain?2. Describe the color of an S. aureus after performing simple stain with Safranin Red. Why?3. What do you conclude after performing simple stain and observing "Purple cells longerthan they are wideGram Stain1. How does the bacterial cell structure contribute to the mechanism of Gram staining?2. What is the mordant in Gram stain and why you need it?3. Why must young cultures be used when doing a Gram stain?Negative Stain1.а.Name the bacterium used in negative stainb. Describe morphologyС.Name the disease caused by this bacterium2. Describe what you observe under microscope in negative staining.

Smear preparation: Spreading the culture on the slide and making a smear is the first step in the…

Answered: Smear Preparation 1. What are two goals…

Smear Preparation 1. What are two goals of a good smear? 2. Why does a bacterial smear need to completely air dry prior to heat fixing? 3. What is the purpose of heat fix step?

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

Smear Preparation and Staining Lab
Smear Preparation
1. What are two goals of a good smear?
2. Why does a bacterial smear need to completely air dry prior to heat fixing?
3. What is the purpose of heat fix step?
Simple Stain
1. What is the difference between simple stain and complex stain?
2. Describe the color of an S. aureus after performing simple stain with Safranin Red. Why?
3. What do you conclude after performing simple stain and observing "Purple cells longer
than they are wide
Gram Stain
1. How does the bacterial cell structure contribute to the mechanism of Gram staining?
2. What is the mordant in Gram stain and why you need it?
3. Why must young cultures be used when doing a Gram stain?
Negative Stain
1.
а.
Name the bacterium used in negative stain
b. Describe morphology
С.
Name the disease caused by this bacterium
2. Describe what you observe under microscope in negative staining.

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