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Standard Tubes Volume of 0.1 Hydroxamate standard Tubes Optical Density (OD) Concentration 0.061 0.078 0.095 1 0.1 0.2 0.4 0.002 3 4 0.004 0.125 0.008 Standard Curve of Optical Density (OD) vs Concentration of Hydroxamate 0.14 0.12 0.1 520 nm

Standard Tubes Volume of 0.1 Hydroxamate standard Tubes Optical Density (OD) Concentration 0.061 0.078 0.095 1 0.1 0.2 0.4 0.002 3 4 0.004 0.125 0.008 Standard Curve of Optical Density (OD) vs Concentration of Hydroxamate 0.14 0.12 0.1 520 nm

EBK A SMALL SCALE APPROACH TO ORGANIC L
EBK A SMALL SCALE APPROACH TO ORGANIC L
4th Edition
ISBN:9781305446021
Author:Lampman
Publisher:Lampman
Chapter93: Refractometry
Section: Chapter Questions
Problem 1P
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Standard Tubes Optical Density (OD) Tubes Volume of 0.1 Concentration Hydroxamate standard 1 0.061 0.1 0.078 0.002 3 0.2 0.095 0.004 4 0.4 0.125 0.008 Standard Curve of Optical Density (OD) vs Concentration of Hydroxamate 0.14 0.12 0.1 0.08 ý = 7.9857x + 0.0618 R = 0.9988 0.06 0.04 0.02 0.001 0.002 0.003 0.004 0.005 0.006 0.007 0.008 0.009 Concentration of Hydroxamate Figure 1: Graph of Standard Curve of Optical Density against the Concentration of Hydroxamate. Hydroxamate Assay Tubes From Fig 1, the equation displayed was y = 7.9857x + 0.0618 and the R2-0.9988. From the equation, y intercept was the optical density and x-intercept was the concentration of hydroxamate. Tubes Optical Density at 520 nm Concentration of 0.103 0.077 0.123 hydroxamate formed 0.0052 0.0019 0.0077 0.0092 6 7 0.135 0.114 0.0065 10 0.065 0.0004 Optical Density at 520 nm
Transcribed Image Text:Standard Tubes Optical Density (OD) Tubes Volume of 0.1 Concentration Hydroxamate standard 1 0.061 0.1 0.078 0.002 3 0.2 0.095 0.004 4 0.4 0.125 0.008 Standard Curve of Optical Density (OD) vs Concentration of Hydroxamate 0.14 0.12 0.1 0.08 ý = 7.9857x + 0.0618 R = 0.9988 0.06 0.04 0.02 0.001 0.002 0.003 0.004 0.005 0.006 0.007 0.008 0.009 Concentration of Hydroxamate Figure 1: Graph of Standard Curve of Optical Density against the Concentration of Hydroxamate. Hydroxamate Assay Tubes From Fig 1, the equation displayed was y = 7.9857x + 0.0618 and the R2-0.9988. From the equation, y intercept was the optical density and x-intercept was the concentration of hydroxamate. Tubes Optical Density at 520 nm Concentration of 0.103 0.077 0.123 hydroxamate formed 0.0052 0.0019 0.0077 0.0092 6 7 0.135 0.114 0.0065 10 0.065 0.0004 Optical Density at 520 nm
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