Can you please help me to complete step by step the Log molecular weight I will appreciate your help ũng the Gei (during second week of lab)1. Pour off Coomassie stain into the designated container.2. Wash gel carefully with water to remove excess stain.3. Add Destain solution. Gently rock gel for one or two hours.Protein Ladder (for trendline graph):MigrationRf=DistanceBand MigrationDye Front Migration0-074F. Analyzing the Gel1. Pour destain into waste container. Add approximately 200mL of water to gel box. Gel maycurl up for a few minutes but will eventually become flat again.2. Place plastic wrap on the surface of the light box. Carefully transfer your gel to the plasticwrap. Take a photograph of your gel for your records and lab report. Completely cover your gelwith a layer of plastic wrap.3. Measure the distance travelled by the dye front. DYE FRONT= 54mm4. Measure in mm (not cm) the distance travelled (or "migration distance") for each protein inyour ladder. Measure from the bottom of the well to each band.5. Use the Excel Tutorial Video on graphing and solving for the kD sizes of the unknowns.(mm)U4aV1722.28.0-1110.1660.2030.3140-407esigطمه و81Measurements for Unknown AMigration Distance (mm)0.759,Molecular Weight(in KD)260 2501007550Rf站2015methods StepsResults10DISCOusion 7 AncaConclussion orkDa--260--100--70-35-15-10Log ofMolecular Weight2.415Estimated Molecular Weight

Proteins are polymers of amino acids linked by peptide/amide bonds by the condensation reaction…

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Science

Biochemistry

Can you please help me to complete step by step the Log molecular weight I will appreciate your help

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Can you please help me to complete step by step the Log molecular weight I will appreciate your help

ũng the Gei (during second week of lab)
1. Pour off Coomassie stain into the designated container.
2. Wash gel carefully with water to remove excess stain.
3. Add Destain solution. Gently rock gel for one or two hours.
Protein Ladder (for trendline graph):
Migration
Rf=
Distance
Band Migration
Dye Front Migration
0-074
F. Analyzing the Gel
1. Pour destain into waste container. Add approximately 200mL of water to gel box. Gel may
curl up for a few minutes but will eventually become flat again.
2. Place plastic wrap on the surface of the light box. Carefully transfer your gel to the plastic
wrap. Take a photograph of your gel for your records and lab report. Completely cover your gel
with a layer of plastic wrap.
3. Measure the distance travelled by the dye front. DYE FRONT= 54
mm
4. Measure in mm (not cm) the distance travelled (or "migration distance") for each protein in
your ladder. Measure from the bottom of the well to each band.
5. Use the Excel Tutorial Video on graphing and solving for the kD sizes of the unknowns.
(mm)
U
4
a
V
17
22.
28.
0-111
0.166
0.203
0.314
0-407
esig
طمه و
81
Measurements for Unknown A
Migration Distance (mm)
0.759,
Molecular Weight
(in KD)
260 250
100
75
50
Rf
站
20
15
methods Steps
Results
10
DISCOusion 7 Anca
Conclussion or
kDa
--260-
-100-
-70
-35
-15
-10
Log of
Molecular Weight
2.415
Estimated Molecular Weight

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