Suppose an unknown sample is too concentrated (the absorbance was off the scale) Explain how you would go about determining the actual concentration of BSA in this tube.

Q: Give the color of the bread mixed with Lugol's iodine solution in these situations before exposure…

A: Lugols iodine test is used as confirmation test for starch. Polyiodide ions react with hellicle…

Q: Give the color of the kamoteng kahoy mixed with Lugol's iodine solution in these situations before…

A: Lugol's iodine contains potassium iodide and iodine in water. It is used for various purposes such…

Q: What volume of the saline solution must be administered to the patient in order to deliver 7.7 g of…

A: In 0.9% Normal saline there are 9 grams of NaCl is present in 1000ml or 1liter Here we have to find…

Q: Calculate the CFU present in 150 mL water sample and b) dilution factor up to 5. Consider the…

A: The serial dilution process is used to calculate the number of microorganisms present in a…

Q: Trace the path of filtered fluid.

A: Urine formation involves three steps  1. Ultrafiltration - In this step, filtration of blood occurs…

Q: Using a diagrammatical presentation show how serial dilution was performed if the final volume in…

A: Serial dilution is very useful process for diluting the sample and it is also helps in the…

Q: Name the light-sensitive analytes. Then, describe how to handle these specimens.

A: Many drugs are light sensitive  they are referred as light sensitive analyte.  They get degraded…

Q: How would you determine the contents of the test tubes if all of the samples were transparent?

A: The study of chemical reactions that occurs within living organisms is called biochemistry. This…

Q: Give the color of the rice mixed with Lugol's iodine solution in these situations before exposure…

A: Iodine solution also known as Lugol’s iodine or aqueous iodine solution.Iodine solutioni is amiof…

Q: To establish a standard curve for a BSA standard curve using Bradford, the spectrophotometer…

A: Proteins are composed of twenty standard amino acids attached together via peptide bonds. These…

Q: Calculate the quantity of glycerin needed to prepare 35-mL of the following ophthalmic solution.…

A: Dexamethasone sodium phosphate is a salt of dexamethasone that is particularly suitable for…

Q: Hew much water must be added to 300 ml of a 5 M aqueous sekution of HCin ander to oblair a cal…

A:

Q: Explain the reason for the use of different medium blanks in adjusting thespectrophotometer prior to…

A: Different medium for the growth of bacteria have different components. Due to presence of different…

Q: In using the centrifuge machine, how will this be remedied if there are only 3 separate liquid…

A: Centrifuges are laboratory equipment that is used to separate components in a mixture based on their…

Q: Why are the tubes sealed with a rubber stopper rather than left open to the atmosphere?

A: A rubber stopper is used to seal the tube opening as well as other laboratory glassware such as…

Q: A 0.897 g sample containing the chloride ion is treated with excess lead(II) nitrate. The…

A: Lead(II) Chloride (PbCl2) is an organic compound which is a white solid under ambient conditions. It…

Q: The aerotolerance test is used to determine the oxygen requirements for bacterial growth.

A: FALSE.

Q: Draw a serial dilution that would give a dilution of 1/153 000 000. Each tube in your dilution…

A: Different concentrations of chemicals are required in the laboratory for various investigations. In…

Q: What is alkaline reversion? Explain why this condition gives a false negative result

A: The microbes that affect the intestine of an individual are referred to as enteric pathogens. The…

Q: What effect, if any, does temperature have upon these measurements? Are there any markings on your…

A: TC and TD marking TC signifies the "to contain" value, which is a set value marked on glassware,…

Q: Why is it that a warm cuvette does not lose any significant heat during the absorbance measurement…

A: An absorption spectrophotometer is defined as a device that is used to calculate the absorbed light…

Q: Calculate the CFU present in 100 mL water sample and b) dilution factor in Tube 5.

A: Answer a - CFU present in 100ml solution=58×104 CFU/ml= 580,000 CFU/ml CFU present in 100ml sample=…

Q: Which of the following is antiseptic and disinfectant? O a. Filtration O b. None O c. Heat O d.…

A: Antiseptic  They are those materials which is use to stop the growth of microorganisms.…

Q: Determine the amount of ascorbic acid in the unknown sample using the standard concentrations and…

A: Given : Absorbance at different concentrations  Unknown concentration(C) = (Test absorbance/Standard…

Q: The spectrophotometer is used to measure absorbance experimentally. Briefly, explain how this is…

A: spectrophotometer is an device that is used for the detection of absorbance of compounds on a…

Q: A patient is receiving an IV solution at a rate of 125ML / hr. How much solution will she receive in…

A: Given, the rate of solution which is given to the patient = 125ml/hr. Which means that in 1 hour,…

Q: If we wanted to measure the BSA absorbance without the Biuret reagent, which wavelength would we use…

A: BSA stands for bovine serum albumin, which is a protein extracted from cows' serum albumin. In lab…

Q: A common procedure of for cleaning the grease trap in a sink is to add a product that contains…

A: Sodium hydroxide is also commonly called caustic soda. It is extremely corrosive in nature as well…

Q: To achieve a hundred fold dilution you would add _____ microliters of sample to the appropriate…

A: The dilution method is used to achieve the desired concentration of the sample in a tube or a…

Q: Outline the acid fast staining technique using a flow chart.

A: ACID FAST STAINING TECHNIQUE : it is the differential staining techniques which was first developed…

Q: (a) What two adjustments can be made to the condenser? (b) What effect do these adjustments have on…

A: Microscopes are optical instruments that use lenses to magnify an object. They help to observe…

Q: calculate the Adjusted Ca2+ concentration if.3 the absorbance of ca2+ sample is 0.9, the absorbance…

A: Absorption spectroscopy is a spectroscopic technique in which we measure the absorption of radiation…

Q: During reflux, what is the most suitable heater setting? How long the liquid needs to be boiled at…

A: Reflux involves the condensation of vapor and returns the condensate to the solution. It is one of…

Q: Compute for the sodium concentration of the patient. Use the same formula in the computation of the…

A: Spectrophotometry measures the absorbance spectrum of a substance. It measures the absorbance…

Q: Figure 1 show the shape of the peaks in gas chromatography that causes loss of column efficiency.…

A: Gas chromatography is a novel analytical technique used in analytical chemistry to separate the…

Q: t what temperature and humidity levels should the Operating Room be maintained at and why?

A: Operating rooms are very crucial sites in a hospital. These rooms require special handling due to…

Q: Principle of Neutralization Tests (NT).

A: In a neutralization test, serum and virus are reacted together in equal volumes and inoculated into…

Q: Why do you think it is important to refrigerate a urine sample if an analysis cannot be performed…

A: Glucose (sugar) in the urine will continue to be used by any bacteria or cells found in a urine…

Q: Chemical formula of the precipitate or positive result of the following: 1. Biuret test 2.…

A: Biuret test, also known as Piotrowski's test, is a chemical test used for detecting the presence of…

Q: The clarity of a urine sample should be determined a. After warming up the sample to 45°C b.…

A: The normal color of urine is pale to dark yellow and it is usually clear with no visible particles.…

Q: The wavelength we used in hemoglobin determination experiment is: 700 nm. O b. 350 nm, C. 540 m. O…

A: Hemoglobin is an iron-containing oxygen-transport globular protein found in virtually all mammals'…

Q: Why do detergent powders removes stain?

A: Answer 1: Detergents have the power to clean. Detergents are surfactants. Detergents are amphiphilic…

Q: What happens to winogradsky column if it is put in a dark place in room temperature.

A: The Winogradsky column is a small, enclosed ecosystem used to enrich sediment microbial communities,…

Q: What volume of a 40% v/v solution must be used to produce 500mL of 5% v/v solution?

A: The concentration of a drug is generally determined by the dilution of the drug. Various types of…

Q: When air is blown from mouth into a test – tube containing lime water, the lime water turned milky…

A: The common name for a dilute aqueous solution of calcium hydroxide is lime water. Lime water is…

Q: What physical factors can influence the activity of a disinfectant

A: Introduction- Disinfection is the process by which we can eliminate the growth of microorganism…

Q: On the graph that relates absorbance to flavin concentration, draw a horizontal line that starts at…

A: Absorbance can be defined as the optical density that means the amount or the quantity of light…

Q: Which molecules, or function groups, or complexes that may be responsible for the absorbance at 595…

A: Spectrophotometry applications are useful to measure the absorbance, reflectance, and transmission…

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.