Table 1: Standard Curve for Bradford Method. Absorbance measured at 595 nm Concentration of BSA Stock solution is 200 µg/mL Amount of protein in tube (ug) O ug Volume of Volume of BSA Absorbance Bradford reagent 3000 µl Cuvette no. Standard 0.00 O µl 30 µl 60 µl B 3000 µl 3000 µl 3000 µl 0.1435 1 0.2968 2 0.402 90 μι 120 µl 150 µl 0.5038 3000 µl 4 0.587 3000 µl LO

Fundamentals Of Analytical Chemistry
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ISBN:9781285640686
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Chapter8: Sampling, Standardization, And Calibration
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Table 1: Standard Curve for Bradford Method. Absorbance measured at 595 nm
Concentration of BSA Stock solution is 200 ug/mL
Amount of protein
in tube (ug)
O ug
Volume of
Volume of BSA
Absorbance
Bradford reagent
3000 µl
3000 µl
3000 µl
3000 µl
3000 µl
3000 µl
Cuvette no.
Standard
0.00
O ul
30 µl
60 μl
90 μl
120 µl
150 µl
В
0.1435
1
0.2968
2
0.402
3
0.5038
4
0.587
Transcribed Image Text:Table 1: Standard Curve for Bradford Method. Absorbance measured at 595 nm Concentration of BSA Stock solution is 200 ug/mL Amount of protein in tube (ug) O ug Volume of Volume of BSA Absorbance Bradford reagent 3000 µl 3000 µl 3000 µl 3000 µl 3000 µl 3000 µl Cuvette no. Standard 0.00 O ul 30 µl 60 μl 90 μl 120 µl 150 µl В 0.1435 1 0.2968 2 0.402 3 0.5038 4 0.587
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