TABLE 5.3 The sequence specificities of some proteolytic enzymes and cyanogen bromide R, O R2 O N-terminal -..-N- C-...C-teminal нн Enzyme Preferred Site Source R, = Lys, Arg From digestive systems of animals, many other sources Trypsin Chymotrypsin R, = Tyr, Trp, Phe, Leu Same as trypsin R, = Arg From blood; involved in coagulation Thrombin R, = Asp, Glu V-8 protease Prolyl endopeptidase Subtilisin From Staphylococcus aureus = Pro Lamb kidney, other tissues Very little specificity From various bacilli Carboxypeptidase A R2 = C-terminal amino acid From digestive systems of animals Thermolysin R2 = Leu, Val, Ile, Met From Bacillus thermoproteolyticus Cyanogen bromide R1 = Met

TABLE 5.3 The sequence specificities of some proteolytic enzymes and cyanogen bromide R, O R2 O N-terminal -..-N- C-...C-teminal нн Enzyme Preferred Site Source R, = Lys, Arg From digestive systems of animals, many other sources Trypsin Chymotrypsin R, = Tyr, Trp, Phe, Leu Same as trypsin R, = Arg From blood; involved in coagulation Thrombin R, = Asp, Glu V-8 protease Prolyl endopeptidase Subtilisin From Staphylococcus aureus = Pro Lamb kidney, other tissues Very little specificity From various bacilli Carboxypeptidase A R2 = C-terminal amino acid From digestive systems of animals Thermolysin R2 = Leu, Val, Ile, Met From Bacillus thermoproteolyticus Cyanogen bromide R1 = Met

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter24: Lipid Biosynthesis

Section: Chapter Questions

Problem 9P

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