Terminators The addition of an excess of ddNTP leads to the stop of DNA replication in bacteria: explain the biochemistry behind this process (hint: the chemical structures of NTP, DNTP and ddNTP are shown below). "o- "o-P-o "o-P-o "o-p-o "o-p-o "o-P-o "o-P-o "o--o Base Base Base CH, CH, CH, H. H. H H. H. он он OH H Ribonucleoside triphosphate NTP) Deoxyribonucleoside triphosphate (dNTP) Dideoxyribonucleoside triphosphate iddNTP)

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter28: Dna Metabolism: Replication, Recombination, And Repair
Section: Chapter Questions
Problem 1P: Semiconservative or Conservative DNA Replication If 15N-Iabeled E. coli DNA has a density of 1.724...
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Terminators
The addition of an excess of ddNTP leads to the stop of DNA replication in bacteria: explain the biochemistry behind this
process (hint: the chemical structures of NTP, DNTP and ddNTP are shown below).
"o-P-o
"0-P-o
"o-P-o
"o-P-o
"O-P-o
"o-P-o
"0-P-o
"o-P-o
-o-P-o
Base
Base
Base
CH,
CH,
CH,
H.
H.
OH OH
ÓH H
Dideoxyribonucleoside
triphosphate
(ddNTP)
Ribonucleoside
triphosphate
(NTP)
Deoxyribonucleoside
triphosphate
(DNTP)
Transcribed Image Text:Terminators The addition of an excess of ddNTP leads to the stop of DNA replication in bacteria: explain the biochemistry behind this process (hint: the chemical structures of NTP, DNTP and ddNTP are shown below). "o-P-o "0-P-o "o-P-o "o-P-o "O-P-o "o-P-o "0-P-o "o-P-o -o-P-o Base Base Base CH, CH, CH, H. H. OH OH ÓH H Dideoxyribonucleoside triphosphate (ddNTP) Ribonucleoside triphosphate (NTP) Deoxyribonucleoside triphosphate (DNTP)
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