The DNA polymerase used in PCR is taken from Thermus aquaticus, a bacterium that thrives in the extremely high temperatures of hot springs. Why is it necessary to use special DNA polymerase? * O Thermus aquaticus is accustomed to efficient replication. Thermus aquaticus can survive at boiling and freezing temperatures, both of which are necessary during PCR. O "Normal" DNA polymerase would denature in the high temperatures used in PCR. O Thermus aquaticus is able to supply a varied amount of bases to the DNA template.

The DNA polymerase used in PCR is taken from Thermus aquaticus, a bacterium that thrives in the extremely high temperatures of hot springs. Why is it necessary to use special DNA polymerase? * O Thermus aquaticus is accustomed to efficient replication. Thermus aquaticus can survive at boiling and freezing temperatures, both of which are necessary during PCR. O "Normal" DNA polymerase would denature in the high temperatures used in PCR. O Thermus aquaticus is able to supply a varied amount of bases to the DNA template.

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 8SQ

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We usually think of enzymes as being most active at around 37°C, yet in PCR the DNA polymerase is subjected to multiple exposures of relatively high temperatures and seems to function appropriately at 65–75°C. What is special about the DNA polymerase typically used in PCR?
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In the very first round of PCR using genomic DNA, the DNA primers prime synthesis that terminates only when the cycle ends (or when a random end of DNA is encountered). Yet, by the end of 20 to 30 cycles - a typical amplification - the only visible product is defined precisely by the ends of the DNA primers. Explain how.
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