The DNA sequence before the protein coding region that determines when a gene is used is called the A regulatory protein that binds DNA to determine whether or not a gene is transcribed is called a Two proteins that make up part of the bacterial flagellum are jointly regulated and transcribed together in a(n) operon is normally transcribed unless it is blocked by a repressor. A(n) operon is normally blocked by a repressor, until it is removed allow transcription.

The DNA sequence before the protein coding region that determines when a gene is used is called the A regulatory protein that binds DNA to determine whether or not a gene is transcribed is called a Two proteins that make up part of the bacterial flagellum are jointly regulated and transcribed together in a(n) operon is normally transcribed unless it is blocked by a repressor. A(n) operon is normally blocked by a repressor, until it is removed allow transcription.

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 9TYU: A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous...

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A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcription
The lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose metabolism. Mutations to various regions of the lac operon have been discovered which affect both the control of gene expression and the function of the gene products. These mutations include: I - = produces a repressor protein that cannot bind to the operator region IS = produces a repressor protein that cannot be removed from the operator region OC = cannot bind a repressor protein Z - = produces a defective (non-functioning) β-galactosidase enzyme + = wild type Detail the functionality of the lac operon under the following genetic and cellular conditions. You do not need to consider the role of CAP in your analysis. Genes Repressed (yes/no) Constitutive (yes/no) IS O+ Z+ IS OC Z+
The lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose metabolism. Mutations to various regions of the lac operon have been discovered which affect both the control of gene expression and the function of the gene products. These mutations include: I - = produces a repressor protein that cannot bind to the operator region IS = produces a repressor protein that cannot be removed from the operator region OC = cannot bind a repressor protein Z - = produces a defective (non-functioning) β-galactosidase enzyme + = wild type Detail the functionality of the lac operon under the following genetic and cellular conditions. You do not need to consider the role of CAP in your analysis. Genes Repressed (yes/no) Constitutive (yes/no) I+ OC Z+ I+ O+ Z+ (Lactose present) I- O+ Z+
In the absence of lactose, the genes of the lac operon are: Not transcribed; because there is no lactose to activate the polymerase Not transcribed; because there is no lactose to inhibit the repressor Transcribed; because there is no lactose to inhibit the repressor Transcribed; because there is no lactose to activate the repressor
A mutation in the operator region of the trp operon can prevent the trp repressor from binding to this operator. When these mutant cells are placed in a solution containing chicken broth, which of the following happens? a. Tryptophan would bind to the repressor. b. The Repressor would bind to the operator irrespective of the presence of amino acids in the environment. c. The transcription of the trip operon would be inhibited. d. The transcription of the repressor protein is inhibited
Given the Table below concerning the lac operon, match the letter with what would be occurring for each scenario. Repressor is bound to the operator, cAMP complex is bound and transcription is prevented. Allolactose is bound to the repressor, cAMP complex is bound, and transcription is prevalent. Allolactose is bound to the repressor, cAMP complex is not assembled, and transcription is slow Repressor is bound to the operator, cAMP complex is not assembled, and transcription is prevented.
A mutation in the repressor LacI causes it to lose the ability to bind its effector, allolactose. What will be the effect of this mutation in the presence of allolactose (assume glucose is absent)? a) LacI cannot bind to the lac operator (lacO) and transcription of the lac operon is blockedb) LacI is permanently bound to the lac operator (lacO) and transcription of the lac operon is blocked c) LacI cannot bind to the lac operator (lacO) and transcription of the lac operon increasesd) LacI is permanently bound to the lac operator (lacO) and transcription of the lac operon increases
Which of the following statements about the trp operon is false? If a mutation in region 2 prevents the formation of the 2 + 3 loop in the 5’UTR transcription levels will increase. The trp operon is an example of negative repressible operon. Formation of the anti-terminator in the 5’UTR of the trp operon occurs when trp levels are low. In the presence of high trp-tRNA, the genes of the trp operon are not expressed. The role of trp is to bind to the repressor protein and activate it.
Which of the following statements is not true of both the LacI and TrpR repressor proteins?a) an allosteric effector molecule changes the DNA-binding activity of the proteinb) transcription is blocked when the repressor protein is bound to the operatorc) the repressor protein is trans-actingd) the allosteric effector causes the repressor to release from the operatore) the operon regulated by the repressor produces the allosteric effector molecule
When iron is scarce, some bacteria can stop synthesis of all enzymes that require iron (Fe3+), such as superoxide dismutase (SOD). Which of the following accurately describes a mechanism for this regulation? Mark all that apply. A. Regulation occurs by an inducible operon B. When absent, the lack of iron causes the repressor to deactivate and block the promoter region for these genes C. Regulation occurs by a repressible operon D. When present, iron binds and activates the repressor protein
In the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan is removed from its co-repressor, causing it to detach from the operator tryptophan is removed from its co-repressor, causing it to attach to the operator tryptophan is bound to its co-repressor, causing it to detach from the operator tryptophan is bound to its co-repressor, causing it to attach to the operator all of the above occur at equal rates when tryptophan levels get too high (Option C is the answer for the question above) What effect will the above process have on the tryptophan operon? RNA polymerase will detach from the promoter, initiating transcription RNA polymerase will detach from the promoter, stopping transcription RNA polymerase will attach to the promoter, initiating transcription RNA polymerase will attach to the promoter, stopping transcription all of the above occur at equal rates when tryptophan levels get too high
In the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan is removed from its co-repressor, causing it to detach from the operator tryptophan is removed from its co-repressor, causing it to attach to the operator tryptophan is bound to its co-repressor, causing it to detach from the operator tryptophan is bound to its co-repressor, causing it to attach to the operator all of the above occur at equal rates when tryptophan levels get too high (Option D is the answer for the question above) What effect will the above process have on the tryptophan operon? RNA polymerase will detach from the promoter, initiating transcription RNA polymerase will detach from the promoter, stopping transcription RNA polymerase will attach to the promoter, initiating transcription RNA polymerase will attach to the promoter, stopping transcription all of the above occur at equal rates when tryptophan levels get too high