The enzymatic reaction rate r is given by equation 2 below as a function of substrate concentration Cs and kinetics parameters KM and rmax. Using linear and nonlinear regression in excel, evaluate the kinetic parameter constants of a biosystem given the gathered data from a series of batch runs

The enzymatic reaction rate r is given by equation 2 below as a function of substrate concentration Cs and kinetics parameters KM and rmax. Using linear and nonlinear regression in excel, evaluate the kinetic parameter constants of a biosystem given the gathered data from a series of batch runs

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter6: Biomedical Electrodes, Sensors, And Transducers

Section: Chapter Questions

Problem 15P

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Question

2. The enzymatic reaction rate r is given by equation 2 below as a function of substrate
concentration Cs and kinetics parameters KM and rmax. Using linear and nonlinear
regression in excel, evaluate the kinetic parameter constants of a biosystem given
the gathered data from a series of batch runs

Substrate Concentration
Initial Reaction Rate
Tmax Cs
r =
kM + Cs
(2)
mmol/L
mmol/L min
0.20
2
0.22
3
0.30
5
0.45
7
0.41
10
0.50
15
0.40
20
0.33

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