An enzymatic reaction was carried out in a batch-operated reactor until exhaustion of the substrate. The formation of the product was monitored over time, obtaining the results shown in Table 1. Answer: a) Knowing that the initial substrate concentration was 0.2 M and that the stoichiometry reaction is: 1S –> 2P, estimate the substrate concentration over time from the data from [P] data in Table 1; b) Determine the parameters of the Michaelis-Menten equation, Vmax and Km, by fitting direct from the hyperbola function; c) Determine the parameters Vmáx and Km, by the linearized equation;
An enzymatic reaction was carried out in a batch-operated reactor until exhaustion of the substrate. The formation of the product was monitored over time, obtaining the results shown in Table 1. Answer: a) Knowing that the initial substrate concentration was 0.2 M and that the stoichiometry reaction is: 1S –> 2P, estimate the substrate concentration over time from the data from [P] data in Table 1; b) Determine the parameters of the Michaelis-Menten equation, Vmax and Km, by fitting direct from the hyperbola function; c) Determine the parameters Vmáx and Km, by the linearized equation;
Chapter2: Decimal Fractions
Section: Chapter Questions
Problem 12PP
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Question
An enzymatic reaction was carried out in a batch-operated reactor until exhaustion of the substrate. The formation of the product was monitored over time, obtaining the results shown in Table 1. Answer:
a) Knowing that the initial substrate concentration was 0.2 M and that the stoichiometry reaction is: 1S –> 2P, estimate the substrate concentration over time from the data from [P] data in Table 1;
b) Determine the parameters of the Michaelis-Menten equation, Vmax and Km, by fitting direct from the hyperbola function;
c) Determine the parameters Vmáx and Km, by the linearized equation;
![t (h)
[P] (M)
0,0
0,8
1,7
2,8
3,7
4,5
5,0
0,39
5,6
0,00
0,08
0,15
0,25
0,30
0,36
0,40](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F0fdea3dd-f249-4dc2-af82-9d8ab2ce8272%2F38c4c733-9ca8-4a4a-ac7b-221a70b66af0%2Fe4hwcb9_processed.png&w=3840&q=75)
Transcribed Image Text:t (h)
[P] (M)
0,0
0,8
1,7
2,8
3,7
4,5
5,0
0,39
5,6
0,00
0,08
0,15
0,25
0,30
0,36
0,40
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