A laboratory in Japan performed a similar experiment on earthworms from different localities of the Japanese archipelago, but instead of linking trypsin activity within worms to anatomical features, they were interested in geographical differences in the ability of the worms to breakdown environmental pollutants using the enzyme polyphenol oxidase. The protocol was similar to the one you used, except that enzyme activity was measured from 100 µl of worm extract. Data obtained are displayed below: Replicate Amount of product liberated over 10 minutes (pmol) Locality Concentration of total protein in worm extract (µg/ml) Hokkaido 1 101 4 2 87 3 3 107 4 4 88 3 89 Hon-shu 1 126 120 3 3 156 4 4 153 119 4 Кyu-shu 1 103 4 99 3 94 6. 4 108 3 110 Okinawa 1 88 3 90 4 3 96 6. 4 100 5 112 3 From the data in the table, calculate the specific activity of polyphenol oxidase (in units of umol/min/mg protein) from each locality, presenting the data as mean + standard error (n=5). You only need to show 1 example of the calculations used Locality Mean Specific activity pmol/ min/mg protein Standard error Hokkaido Hon-shu Кyu-shu Okinawa

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
icon
Concept explainers
Question
A laboratory in Japan performed a similar experiment on earthworms from different localities
of the Japanese archipelago, but instead of linking trypsin activity within worms to anatomical
features, they were interested in geographical differences in the ability of the worms to
breakdown environmental pollutants using the enzyme polyphenol oxidase. The protocol was
similar to the one you used, except that enzyme activity was measured from 100 µl of worm
extract. Data obtained are displayed below:
Replicate Amount of product
liberated over 10
minutes (pmol)
Locality
Concentration of total
protein in worm extract
(ug/ml)
Hokkaido
1
101
4
87
3
3
107
4
4
88
3
89
Hon-shu
1
126
2
120
3
156
4
4
153
5
119
4
Кyu-shu
1
103
4
2
99
3
94
4
108
3
110
Okinawa
1
88
3
2
90
4
3
96
6.
4
100
112
3
From the data in the table, calculate the specific activity of polyphenol oxidase (in units of
umol/min/mg protein) from each locality, presenting the data as mean + standard error (n=5).
You only need to show 1 example of the calculations used
Locality
Mean Specific activity µmol/ min/mg protein
Standard error
Hokkaido
Hon-shu
Kyu-shu
Okinawa
Transcribed Image Text:A laboratory in Japan performed a similar experiment on earthworms from different localities of the Japanese archipelago, but instead of linking trypsin activity within worms to anatomical features, they were interested in geographical differences in the ability of the worms to breakdown environmental pollutants using the enzyme polyphenol oxidase. The protocol was similar to the one you used, except that enzyme activity was measured from 100 µl of worm extract. Data obtained are displayed below: Replicate Amount of product liberated over 10 minutes (pmol) Locality Concentration of total protein in worm extract (ug/ml) Hokkaido 1 101 4 87 3 3 107 4 4 88 3 89 Hon-shu 1 126 2 120 3 156 4 4 153 5 119 4 Кyu-shu 1 103 4 2 99 3 94 4 108 3 110 Okinawa 1 88 3 2 90 4 3 96 6. 4 100 112 3 From the data in the table, calculate the specific activity of polyphenol oxidase (in units of umol/min/mg protein) from each locality, presenting the data as mean + standard error (n=5). You only need to show 1 example of the calculations used Locality Mean Specific activity µmol/ min/mg protein Standard error Hokkaido Hon-shu Kyu-shu Okinawa
Expert Solution
steps

Step by step

Solved in 3 steps with 2 images

Blurred answer
Knowledge Booster
Enzyme kinetics
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education