As a reference model, a 7 L baffled stirred-tank reactor with a 3:1 (H:D) aspect was employed to scale down to a 7 mL tiny bioreactor. Dt = 0.16 m; Di = 0.07 m; N = 100 rpm are the dimensions of the huge tank. 1)Using geometric similarities, determine the dimensions of the micro bioreactor (Dt, Di, and H).
Q: If Vmax for a reaction is 10 uM · s' and the KM is 0.5 µM, what is the reaction velocity when the…
A: Michaelis-Menten equation defines the rate of enzyme catalysis by relating the rate of reaction…
Q: Using conversion factors, solve each of the following clinicalproblems:a. A nurse practitioner…
A: Ans: Given values: Stock of promethazine injection : 25mg/mL Dose required : 12.5 mg Ampicillin…
Q: Describe the effects of a lethal dose of ionizingradiation at the molecular level. What type of…
A: Hello. Since you have posted multiple questions and not specified which question needs to be solved,…
Q: The following Rf values were computed from a reverse phase chromatography experiment. Arran them…
A: There are different techniques to separate a compound from a mixture of substances. The different…
Q: 1. What is a “dNTP” and why are they included in the reaction mixture? 2. What will be the final…
A: dNTP is the acronym used for deoxynucleoside triphosphate. dNTPs are most commonly used in the…
Q: During cell couting, when the data are plotted in an ordinary graph or on a cross-section paper, why…
A: For experiments involving growth of cells whether bacterial or eukaryotic the results of a growth…
Q: Using conversion factors, solve each of the following clinical problems:a. The physician has ordered…
A: Since you have posted a question with multiple sub-parts, we will solve first three sub-parts for…
Q: 8- 2- 315 405 [Substrate] (nM) 45 90 135 180 225 270 360 450 495 540 58! What is the Vmax of the…
A: Michaelis Menton kinetics: This is well known model for Enzyme kinetics. This model relates rate of…
Q: Calculate the dimensionless spray flux for the normal case and the atypical batch, and explain why…
A: As given in the question, the value of spray flux for: Normal value - 0.52 Atypical value - 1.46…
Q: What mode of chromatographic separation (RPLC, NPLC, HILIC, SEC, IEX) is best used for the specified…
A: Chromatography is an analytical method used in chemical analysis to separate a mixture into its…
Q: A chromatographic separation of four compounds gave the following results. The dead time (tm) is…
A: Chromatography is a technique for separating a mixture in the laboratory. The combination is…
Q: True or False. If drug A produces a larger zone of inhibition than drug B on the Kirby-Bauer disk…
A: Introduction If drug A does produce a larger zone of inhibition, that is one positive attribute of…
Q: Would you expect a change in column temperature to have an effect on a reversed-phase…
A: Reversed-phase chromatography (RPC) technique use hydrophobic stationary phase to separate…
Q: An enzymatic reaction was carried out in a batch-operated reactor until exhaustion of the substrate.…
A: Michaelis-Menten equation is used for analyzing the rate of the enzyme-catalyzed reactions in a…
Q: An enzyme is present at 100 nM (nanomolar) and has a Vmax value of 25 uM/s (micromolar/second). The…
A: Michaelis–Menten kinetics is a well established model of enzyme kinetics. The Michaelis -Menten…
Q: The data in the table below were collected during a laboratory session assaying for the kinetics of…
A: Acid phosphatase is a lysosomal enzyme released by many cells/tissues type. Some cells release high…
Q: An enzyme is present at 100 nM (nanomolar) and has a Vmax value of 25 uM/s (micromolar/second). The…
A: Enzymes are usually protein molecules which catalyzes several biochemical reactions in our body…
Q: USING PARMESAN CHEESE, DESCRIBE A BIOREACTOR SUITABLE FOR THE PROCESS? What is the reactor…
A: A bioreactor for this process is cylindrical in shape with eight valves along the top where gases…
Q: Calculate the volume of BSA stock that will be required to make the standard solutions needed to…
A: A stock solution is a concentrated solution that is diluted to make a working solution. It is used…
Q: When [Et] = 20 nM and [SAD] = 40 µM, the reaction velocity, V0, is 9.6µM s1. Calculate Km for the…
A: Michaelis menten constant, Km is the substrate concentration required to produce half maximum…
Q: 7.1 The following results were obtained for an enzyme-catalysed reaction Substrate concentration…
A: The enzyme is biomolecule that can act as catalyst in the biochemical increasing the rate of…
Q: Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors…
A: A bioreactor is a man-made device specially designed for supporting biologically active environment.…
Q: calculate the reaction velocity at saturating substrate concentrations. Your numerical answer is…
A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…
Q: Explain what method is used to identify the solid structure of the catalyst (catalyst…
A: The characterization of many catalysts involves a knowledge of the physical properties of the…
Q: To purify a given enzyme from a crude extract that contains it, fractional precipitations, exchange…
A: Protein purification is necessary to purify the protein to homogeneity. Homogeneous protein sample…
Q: Using pearson's square, how to attain 30o Brix syrup using 50oBrix and 20o Brix syrup solutions?
A: Here, the given two available syrups are 50°Brix and 20°Brix, that is one of the solutions is higher…
Q: eluent strength increase as solvent becomes less polar in reversed-phase chromatography, whereas…
A: Reversed- phase chromatography – uses a non - polar stationary phase. Eluent strength is increased…
Q: You want to feed a new flask @: 2 x 105 cells/mL, 8 mL total per flask. Describe how you dilute your…
A: Viable or the live cells can be counted by the instrument called the hemocytometer. These live cells…
Q: A 0.578-g saltwater sample was titrated with 1.864 g AgNO3 solution to reach the endpoint of the…
A: AgNO3 and saltwater titration Weight of AgNO3 = 5g In 100 ml water solution is made. 1.864 g AgNO3…
Q: Explain briefly effects and importance of following phenomena on bioreactor design a) oxygen demand…
A: Answer: BIOREACTOR = It is the system which supports biologically active environment. It is a kind…
Q: In order to sediment mitochondria, you were asked to centrifuge at an RPM of 5600 to give an RCF of…
A: The relative centrifugal force (RCF) is the radial force that is generated in a spinning rotor that…
Q: If we want to observe the following reaction, what must we add to a test tube? Select all that…
A: A chemical reaction is defined as the interaction between different molecules.
Q: The following results were obtained for an enzyme-catalysed reaction Substrate concentration (mmol…
A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that…
Q: Enzyme X has a molecular weight of 48,000. It converts substrate Z into product Y. Z absorbs at 340…
A:
Q: What is the chemistry principle behind when (treatment: heavy cream) is subjected to room…
A: Heavy cream temperature is important for the physical characteristics of the cream. At room…
Q: How to prepare a 12 mL solution of 1 mM NaCl in deionized water if your precision balance has a…
A: Molality or molar concentration is the amount if substrate dissolved in a certain mass of solvent.…
Q: For an exponentially growing culture that increases from 5 *106cells/ml to 5 * 108cells/ml in 8 h,…
A: Bacteria are microorganism that most commonly occur in the soil, air, water and in adverse…
Q: When a mixture of various molecular sized dyes are run through a gel chromatography column, what is…
A: Chromatography is a process of separation of substances from mixtures based on their molecular size.…
Q: The Lineweaver-Burke plots of a reaction without inhibitor and one with non-competitive inhibitor…
A: Enzymes are catalysts that enhance the rate of biochemical reactions.
Q: Imagine you will use a centrifuge in the laboratory indicating rpm and your protocol requires…
A: Centrifuge Device: A centrifuge is a laboratory apparatus that separates fluids, gaseous or…
Q: Using pearson's square, how to attain 30o Brix syrup using 50oBrix and 20o Brix syrup solutions?…
A: For using persons square we need to make a square first then on the left side of the square we write…
Q: 7.1 The following results were obtained for an enzyme-catalysed reaction Substrate concentration…
A: According to Michaelis-Menten Kinetics, when the rate or velocity of an enzyme catalyzed reaction…
Q: The decomposition reaction of atmospheric peroxyacetylnitrate (PAN) to products was studied, and the…
A: Arrhenius equation K = Ae-E/RT ln K = lnA +(-E/R)(1/T) Slope = lnK/(1/T) Y-intercept = lnA K1 =…
Q: If the enzyme lactase has a Vo of 0.40 mM per minute when [S] = 1.25 mM, and a Vo of 1.0 mM per…
A: The Lineweaver-Burk equation is obtained by taking the reciprocal of the Michaelis-Menten equation.…
Q: For the nitrate reduction test, complete the table below Reaction Gas evolution (Yes/No) Color…
A: Nitrate Reduction Test -- Introduction -- Oxygen is present in the atmosphere as O2 which function…
Q: For the same chromatographic column and experimental conditions Rs when H = 3 cm = Rs when H = 5 cm…
A: Asked : For same chromatographic column and experiment conditions the answer out of given options is…
As a reference model, a 7 L baffled stirred-tank reactor with a 3:1 (H:D) aspect was employed to scale down to a 7 mL tiny bioreactor.
Dt = 0.16 m; Di = 0.07 m; N = 100 rpm are the dimensions of the huge tank.
1)Using geometric similarities, determine the dimensions of the micro bioreactor (Dt, Di, and H).
Step by step
Solved in 3 steps
- In kool aid chromatography, what factors affect the efficiency of the column? Also discuss the Van-Deemter equation.Imagine you will use a centrifuge in the laboratory indicating rpm and your protocol requires 3500xg, what would your setting be in a given centrifuge with a radius of 10cm? Provide computationsIf a data from an enzyme experiment is plotted as a Lineweaver - Burk plot, and the Vmax is 0.02 sec/mol, and x-intercept is -2.5 mM-1, then what is the KM value?
- Plot a line weaver-burk graph for Km=6.30mM and Vmax=360uM/min when an experiment has 5 tubes with concentrations of substrates of 1.0mM, 10mM, 50mM, and 100mM.If the data from an enzyme experiment is plotted as a Lineweaver-Burk plot, and the Vmax is 0.02 mol/sec, and x-intercept is –2.5 mM then what is the KM value? Show yourwork/reasoning.Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.
- Why does eluent strength increase as solvent becomesless polar in reversed-phase chromatography, whereas eluentstrength increases as solvent becomes more polar in normal-phase chromatography? What kind of gradient is used in supercritical fluid chromatography?Given the following set of kinetic data, what is the KM of the enzyme? Numerical answer, only (assuming the same units as seen in the table). [S] (micro M) vo (Micro mol/sec) 1 49 2 96 8 249 50 498 100 656 1000 778 5000 999Using pearson's square, how to attain 30o Brix syrup using 50oBrix and 20o Brix syrup solutions? (please indicate the solution and formula)
- For some Enzyme, the Vmax is 18 micromols/min, Km is 400 microM. If the substrate concentration is 100 microM, what is the velocity of the reaction?if the absorbance of pure chlorophyll a in a 1 cm cell is 0.876 at 530 nm and the concentration is 8.66 x 10^-6 M, what is the molar extinction coefficient of chlorophyll a?Given the following information about an enzymatic reaction, calculate the initial velocity. Your numerical answer is assumed to have the units of nM sec-1. Vmax = 5 nM sec-1 KM = 1 mM [S] = 0.25 mM