You have performed an experiment and your aim is to obtain pure colonies. However, you observe some colony variations and you need to verify that this is not due to contamination, name and describe the staining method would you use to achieve this goal?
Q: The first step in identifying an organism from a pure working culture is to perform a(n) __________…
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Q: shape
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A: Ans: Lugols solution: The Lugols solution is 2.5 to 25% weight by volume total iodine.
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Q: List the step-by-step procedure of Gram staining.
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Q: 5. You receive a bacterial culture in the lab and your job is to figure out information which will…
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Q: In acid fast staining, lipoidal material of acid fast cells absorbs the methylene blue. Upon the…
A:
Q: Briefly, in your own words, describe the steps of the gram staining procedure.
A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…
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A: Bacteria are microscopic organisms which belong to prokaryote because these are unicellular…
Q: What staining method would you use to verify that the isolate is not producing survival structures…
A: Staining is the procedure that helps to visualize the minute organisms under a microscope using…
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A: Given Values: Volume of the 16 hour culture = 500 μl Final Volume of the culture media = 5 ml
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- Briefly, in your own words, describe the steps of the gram staining procedure.Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram stain on a sample before prescribing an antibiotic?In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?
- Which of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.You look at a slide you prepared and, despite the fact that you know you put organism onto the slide in the first place, no organism remains on the slide at the end of the methylene blue staining. Identify TWO different problems in staining that could lead to this outcome.In gram staining, could colors other than red be used as a counterstain?
- why are the steps in gram staining are so carefully standardized?What staining method would you use to verify that the isolate is not producing survivalstructures against unfavourable conditions? Describe the procedure as wellDescribe one error in the staining procedure that would result in a Gram-positive bacterium appearing pink.
- Why should a young culture be used in gram staining rather than an old culture?b. Why is it necessary to decolorize only for a few secondsExplain the principle of Gram staining. (You do not need to describe the procedure step by step).The first step in identifying an organism from a pure working culture is to perform a(n) __________ test. acid-fast staining negative staining endospore staining simple staining Gram stain