The following data describe the catalysis of cleavage of peptide bonds in small peptides by the enzyme elastase. Substrate Ka(mM) ka(s") PAPAIG 4.0 26 PAPA JA 1.5 37 PAPA ĮF 0.64 18 The arrow indicates the peptide bond cleaved in each case. (a) If a mixture of these three substrates was presented to elastase with the concentration of each peptide equal to 0.5 mm, which would be digested most rapidly? Which most slowly? (Assume enzyme is present in excess.) (b) On the basis of these data, suggest what features of amino acid sequence dictate the specificity of proteolytic cleavage by elastase. (c) Elastase is closely related to chymotrypsin. Suggest two kinds of amino acid residues you might expect to find in or near the active site.

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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The following data describe the catalysis of cleavage of peptide bonds in
small peptides by the enzyme elastase.
Substrate
Ka(mM) ka(s")
PAPAIG
4.0
26
PAPA JA
1.5
37
PAPA ĮF
0.64
18
The arrow indicates the peptide bond cleaved in each case.
(a) If a mixture of these three substrates was presented to elastase with the
concentration of each peptide equal to 0.5 mm, which would be digested
most rapidly? Which most slowly? (Assume enzyme is present in excess.)
(b) On the basis of these data, suggest what features of amino acid sequence
dictate the specificity of proteolytic cleavage by elastase.
(c) Elastase is closely related to chymotrypsin. Suggest two kinds of amino
acid residues you might expect to find in or near the active site.
Transcribed Image Text:The following data describe the catalysis of cleavage of peptide bonds in small peptides by the enzyme elastase. Substrate Ka(mM) ka(s") PAPAIG 4.0 26 PAPA JA 1.5 37 PAPA ĮF 0.64 18 The arrow indicates the peptide bond cleaved in each case. (a) If a mixture of these three substrates was presented to elastase with the concentration of each peptide equal to 0.5 mm, which would be digested most rapidly? Which most slowly? (Assume enzyme is present in excess.) (b) On the basis of these data, suggest what features of amino acid sequence dictate the specificity of proteolytic cleavage by elastase. (c) Elastase is closely related to chymotrypsin. Suggest two kinds of amino acid residues you might expect to find in or near the active site.
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