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The given protein, Protein X, is a heterotrimer - meaning it is a multimeric protein consisting of different polypeptide chains. Its molecular weight is 200 kDa.  Using SDS-PAGE, the protein was characterized and the following profile is the result (attached in the picture).   1: Standard Protein Ladder 2: Protein X solution 3: Protein X solution + β-mercaptoethanol   Based on the given gel profile in the picture, can it be concluded that the solution of Protein X is pure? Explain. can it be concluded that disulfide bonds hold together Protein X's quaternary structure? Explain.

The given protein, Protein X, is a heterotrimer - meaning it is a multimeric protein consisting of different polypeptide chains. Its molecular weight is 200 kDa.  Using SDS-PAGE, the protein was characterized and the following profile is the result (attached in the picture).   1: Standard Protein Ladder 2: Protein X solution 3: Protein X solution + β-mercaptoethanol   Based on the given gel profile in the picture, can it be concluded that the solution of Protein X is pure? Explain. can it be concluded that disulfide bonds hold together Protein X's quaternary structure? Explain.

Biomedical Instrumentation Systems
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter6: Biomedical Electrodes, Sensors, And Transducers
Section: Chapter Questions
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The given protein, Protein X, is a heterotrimer - meaning it is a multimeric protein consisting of different polypeptide chains. Its molecular weight is 200 kDa.  Using SDS-PAGE, the protein was characterized and the following profile is the result (attached in the picture).

 

1: Standard Protein Ladder

2: Protein X solution

3: Protein X solution + β-mercaptoethanol

 

Based on the given gel profile in the picture,

  • can it be concluded that the solution of Protein X is pure? Explain.
  • can it be concluded that disulfide bonds hold together Protein X's quaternary structure? Explain.
200 kDa 150 kDa 100 kDa 75 kDa 50 kDa 25 kDa 1 2 3 |||
Transcribed Image Text:200 kDa 150 kDa 100 kDa 75 kDa 50 kDa 25 kDa 1 2 3 |||
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