The protein known as tyrosinase is needed to make certain types of pigments. Tyrosinase is composed of a single polypeptide with 511amino acids. The molecular mass of this protein is approximately61,300 Da, or 61.3 kDa. People who carry two defective copies ofthe tyrosinase gene have the condition known as albinism. They areunable to make pigment in the skin, eyes, and hair. Western blottingis used to detect proteins that are translated from a particularmRNA. This method is described in Chapter 20 and also in experimentalquestion E4. Skin samples were collected from a pigmentedindividual (lane 1) and from three unrelated albino individuals(lanes 2, 3, and 4) and subjected to a Western blot analysis using anantibody that recognizes tyrosinase.
The protein known as tyrosinase is needed to make certain types of pigments. Tyrosinase is composed of a single polypeptide with 511
amino acids. The molecular mass of this protein is approximately
61,300 Da, or 61.3 kDa. People who carry two defective copies of
the tyrosinase gene have the condition known as albinism. They are
unable to make pigment in the skin, eyes, and hair. Western blotting
is used to detect proteins that are translated from a particular
mRNA. This method is described in Chapter 20 and also in experimental
question E4. Skin samples were collected from a pigmented
individual (lane 1) and from three unrelated albino individuals
(lanes 2, 3, and 4) and subjected to a Western blot analysis using an
antibody that recognizes tyrosinase.
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