The results of the diagram (Agarose electrophoresis photodocumentation andquantification table) correspond to the information for 4 samples of genomic DNAextracted from plant tissue. With which sample (s) could a PCR be performed toamplify a 100 pb region?Al A2АЗ А4IDDNA260 nm 260 nm(pb)10,000sample (ng/uL) 280 m 230 nmA11701.92.0А21801.51.55,000АЗ1701.71.5А41701.92.01,000500100choose the best option:а. Allb. Noneс. А2d. A1, A2е. А1

Agarose gel electrophoresis is the separation technique based upon the size of the macromolecule. in…

Answered: The results of the diagram (Agarose…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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The best combination of regeants for a typical PCR reacion would include the following concentration of reagents: a. 1x buffer, 1.5 mMm MgCl2, 0.5nM primers, 10mMdNTPs, 2units Taq, 1010 copies of template b. none of these answers c. 1x buffer, 1.5 mMm MgCl2, 0.5pM primers, 10mMdNTPs, 2units Taq, 105 copies of template d. 1x buffer, 1.5 mMm NH3 Cl2, 0.5pM primers, 10mMdNTPs, 2units Taq, 102 copies of template e. 1x buffer, 1.5 mMm KCL2 , 0.5nM primers, 10mMdNTPs, 2units Taq, 103copies of template thank you!!
What could you conclude from PCR/DGGE analysis of a samplethat yielded one band by PCR and one band by DGGE? Oneband by PCR and four bands by DGGE?
Entire sequence below needs to beamplified by PCR and subcloned into a plasmid vector. Which of the primersequences listed underneath is the correct reverse primer (6 marks)? Copy correctsequence into your answer. Why primer e) is not the right answer (4 marks)? 5'ATCTCTATTTAATATTTATGTCTATTTAAGCCTCATATTTAAAGACAGGGAAGAGCAGAACGGAGCCCCAGGCCTCTGTGTCCTTCCCTGCATTTCTGAGTTTCATTCTCCTGCCTGTAGCAGTGAGAAAAAGCTCCTGTCCTCCCATCCCCTGGACTGGGAGGTAGATAGGTAAATACCAAGTATTTATTACTATGACTGCTCCCCAGCCCTGGCTCTGCAATGGGCACTGGGATGAGCCGCTGTGAGCCCCTGGTCCTGAGGGTCCCCACCTGGGACCCTTGAGAGTATCAGGTCTCCCACGTGGGAGACAAGAAATCCCTGTTTAATATTTAAACAGCAGTGTTCCCCATCTGGGTCCTTGCACCCCTCACTCTGGCCTCAGCCGACTGCACAGCGGCCCCTGCATCCCCTTGGCTGTGAGGCCCCTGGACAAGCAGAGGTGGCCAGAGCTGGGAGGCATGGCCCTGGGGTCCCACGAATTTGCTGGGGAATCTCGTTTTTCTTCTTAAGACTTTTGGGACATGGTTTGACTCCCGAACATCACCGACGCGTCTCCTGCTG 3'a) 5' TTCCGGAAGAAGCTTATACGG 3'b) 5' CTGTGTTCACCTAATATTCCT 3'c) 5' CAGCAGGAGACGCGTCGGTGA 3'd) 5' AGGAATATTAGTATAATCCAC 3'e) 5' GACGCGTCGGTGATGTTCGGG 3’f) 5'…
A student is trying to add 9.0 pmol of primer mix to a 20.0 µL PCR. The primer mix is at a concentration of 50.0 µM, and the student determines that a serial dilution is required because directly adding the primer mix would require a volume less than 1.00 µL. The student takes 1.40 µL of the primer mix stock and mixes it with 25.0 µL of ultrapure H2O. Calculate the volume (in µL) of the intermediate solution required to add the correct amount of primer mix to the PCR.
If PCR is unsuccessful, how do you adjust the following parameters and why would you adjust them that way:a. MgCl2b. dNTPsc. Primersd. Taqe. Annealing Tempf. Annealing durationg. Extension duration
A region of the genome is amplified by PCR to analyze two closely linked SNPs. PCR products from individual A were labelled with a red-fluorescing dye and those from individual B labelled with a green-fluorescing dye. These PCR products were mixed and hybridized to an oligonucleotide microarray as shown in the figure below. What can we conclude about the data shown? Select all true: a. individual A is heterozygous for the M1 and M2 haplotypes, individual B is heterozygous for M4 and M5 b. individual A is heterozygous for the M1 and M2 haplotypes, individual B is heterozygous for M3 and M6 c. Sanger sequencing traces of these PCR products would show the same double peaks at the same positions for these two individuals d. analyzing this PCR amplified region with conventional Sanger sequencing would be more accurate than the microarray analysis e. individuals A and B could be siblings who share a parent that is homozygous for one of the haplotypes {urgent}
The final amount of each primer required in a PCR reaction is 25 picomol. If the total volume of the PCR reaction is equal to 100 µl and the stock concentration of each primer is equal to 0.0025 mM. Calculate the volume of stock primer that needs to be added in order to ensure a primer amount of 25 picomol.
Match the following terms with their definitions and label each component of the PCR mixture in the diagram (use the letters A-D):I. DNA polymeraseII. PrimersIII. NucleotidesIV. Genomic DNA template A. DNA that contains the target sequence that will be replicated using PCR.B. An enzyme that copies the DNA sequence.C. A mixture of 4 nucleotides (A,G,C, and T) that will be polymerized into the replicated DNA sequence.D. A short DNA sequence that allows the enzyme to bind and initiate polymerization.
The Tm of primers is critical to PCR design. For the primers belowwhich has the lower Tm ? Why? Choose one primer and one reason a.CGATOGTACGATCGTAGCATCGAb. TGO CGATGCTACGAT c. The primer is longer, therefore the Tm is lower due hydrogen bonds. d. The GC is higher (has more GC base pairs ) therefore the TM is lower due more hydrogen e. The AT content is higher has more AT )the Tm is lower due to more hydrogen bonds f. The primer is shorter and therefore has fewer hydrogen bonds
Below is the result of PCR products running on agarose gel electrophoresis. You expect to see one band, but the results show two bands, as shown below. Why is there a lower band? What are they? How can we avoid such a band?
The gel below shows results for the bitter tasting SNP analysis. Analyze the results and annotate the gel to indicate the bitter tasting ability and homozygous/heterozygous status of each of the individuals represented on the gel. There are three lanes of markers, and the first non-maker lane on the left of the gel is an uncut control PCR product (i.e. there are a total of 10 individuals on the gel starting in lane 3).
What phase of PCR (exponential, linear, or stationary) is analyzedto quantitate the amount of DNA or RNA in a sample? Explainwhy this phase is chosen.

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The results of the diagram (Agarose electrophoresis photodocumentation and quantification table) correspond to the information for 4 samples of genomic DNA extracted from plant tissue. With which sample (s) could a PCR be performed to amplify a 100 pb region?